PhD student - Laboratory of Molecular Pathogenetics

Laboratory of Molecular Pathogenetics of the Institute of Biotechnology of the Czech Academy of Sciences located in the BIOCEV research centre in Vestec by Prague announces a selection procedure in accordance with the Act No. 283/1992 Coll. on the Czech Academy of Sciences, and the Statutes of the Czech Academy of Sciences for PhD student position to work on innovative projects regarding different ways in which epigenetic signals and transcription factor networks govern self-renewal, cell specification, differentiation, and reprogramming - a crucial step for advances in cell-based therapy and perspectives for medicine.

Using mouse models, we aim to investigate how disruption of ISL1 and NEUROD1 transcription networks affect epigenetic landscapes and downstream targets during embryonic development. We will use Cre/loxP and Crispr/Cas9 technologies, global gene expression profiling (RNA sequencing), CUT&Tag chromatin profiling, FACS, confocal microscopy, immunohistochemistry, and in situ hybridization to evaluate embryonic phenotypes.

The Laboratory of Molecular Pathogenetics is seeking a highly motivated, hard-working PhD student. The candidate should hold a master’s degree or an equivalent degree in cell biology, genetics, biomedicine, molecular biology, or related sciences. Additionally, the candidate must be willing to work with murine models.

Who are we:

Using genetically modified mouse models and gene expression profiling, we identify molecular targets for the development of preventive and diagnostic strategies.

Our focus:

• transcriptional regulation in neurosensory development and maintenance in the inner ear model
• the molecular mechanisms involved in pathologies associated with diabetes mellitus
• the molecular causes of abnormal embryonic development

More information can be acquired from our website .

Related publications:

1. Filova I et al. Combined Atoh1 and Neurod1 Deletion Reveals Autonomous Growth of Auditory Nerve Fibers. Molecular Neurobiology. 2020, 57: 5307–5323.

2. Bohuslavova R et al. HIF-1 is required for development of the sympathetic nervous system. PNAS, 2019 116 (27) 13414-13423.

3. Gao et al. Pioneering function of Isl1 in the epigenetic control of cardiomyocyte cell fate. Cell Research (2019) 29:486–501.

4. Matsuda et al. Pioneer Factor NeuroD1 Rearranges Transcriptional and Epigenetic Profiles to Execute Microglia-Neuron Conversion. Neuron, 2019, 101, 472–485.

5. Bohuslavova et al. NEUROD1 Is Required for the Early α and β Endocrine Differentiation in the Pancreas. Int J Mol Sci. 2021, 22(13):6713.