Especially in higher eukaryotes, during and following translation, proteins can be modified in highly diverse ways. A very frequently occurring protein modification is the acetylation of a protein’s amino-terminus (N-terminus). While this modification changes the biophysical parameters of a protein’s N-terminus, surprisingly little is known about the functional consequences, if any, of this modification for the grand majority of affected proteins. Recent studies indicated the possibility that an N-terminal acetyl group can affect protein folding and thus protein conformation.
In this project we will first improve a mass spectrometry-based method for assessing protein conformation on a cellular scale. This method will then be applied to the proteomes of human cell lines that model human diseases. Selected proteins with altered conformation will be further validated using other molecular technologies by which we hope to get more insights in protein folding diseases, amongst others.
Our aim is thus to evaluate the influence of the absence or reduction of N-terminal acetylation on a proteome’s conformation.
Desirable but not required (optional):
- Assets are experience with mass spectrometry-based proteomics, data analysis or cloning.
A research project has already been granted to secure your position for 4 years, the typical time needed to successfully complete a PhD-project at VIB and Ghent University. Yet, you will be challenged to obtain your own funding via the abovementioned FWO channel, with full support of the Gevaert lab and the VIB-UGent Center for Medical Biotechnology (https://cmb.vib.be/ ).
This project is part of a long-term collaboration with the Arnesen lab at Bergen University (Norway; https://www.uib.no/rg/nat ) and frequent exchange of data and new ideas, and a possible research stay abroad are foreseen.
You will work in a top-notch lab environment with immediate access to state-of-the-art mass spectrometry via the VIB Proteomics Core (https://connect.vib.be/facilities/proteomics-core ) housed in our Center.
Full career guidance and doctoral training programs are available from the lab, VIB and Ghent University.
How to apply
Motivated candidates are asked to apply online.
Ph D Position In Cellular Biochemistry In The Field Of Proteostasis And Phase Separation, CNRS, Suriname, 23 days ago
To this end, the student will take advantage of the excellent scientific environment of the I2BC and CNRS campus. This integrative study will make use of cellular biochemistry, quantitative proteo...
Ph D Position In Epigenetics And Gene Regulation (1.0 Fte), Utrecht University, Netherlands, 6 days ago
A multitude of DNA sequence-dependent and -independent interactions between transcriptional regulators and the genome are required for tissue-specific gene expression and cell identity. Interferen...
Ph D Position In Mass Spectrometry Imaging, Maastricht University, Netherlands, about 7 hours ago
PhD Position in Mass Spectrometry Imaging PhD Position in Mass Spectrometry Imaging Published Deadline Location 30 Sep 16 Oct Maastricht A fully funded PhD studentship in the field of mass spectro...
Ph D Student, University of A Coruña, Spain, 4 days ago
Grant/funding reference: 851179 Job title: Supramolecular engineering of biologically inspired peptide nanostructures - SENSE Research line / Scientific-technical services: Peptide materials Tasks...
Ph D Candidate In Nutritive Proteins With Functional Properties From Alternative Sources, IRTA, Spain, 13 days ago
Animal welfare, environmental and health concerns are pushing consumers towards replacing meat from animal origin with proteins from alternative sources. Indeed, it is expected that novel vegan me...
Ph D Position (4 Years) At The Department Of Biomedicine, University of Bergen, Norway, 4 days ago
PhD position At the Faculty of Medicine, Department of Biomedicine, a full-time temporary position as PhD candidate is available for four (4) years or max 4 months after completed the PhD degree, ...