TDP-43 aggregation is the major hallmark of nearly all amyotrophic lateral sclerosis (ALS) forms and half of frontotemporal dementia (FTD) cases. Two leading labs are teaming up and combining their expertise to decipher the molecular underpinnings of TDP-43 aggregation. The Da Cruz lab has innovative cellular systems that recapitulate human TDP-43 pathology as seen in patients and the SWITCH lab has unraveled the importance of heterotypic interactions in amyloid proteins. These tools will be powerfully combined in this project to investigate specific (heterotypic) interactions between the aggregation-prone regions of TDP-43 and the background proteome and to determine which cells are most sensitive to TDP-43 aggregation.
This hypothesis will be tested by performing a genome-wide CRISPR and compound screen in a reporter cell line and followed by computational modelling. The most promising hits will then be validated using a combination of biophysical and biochemical techniques as well as cellular functional assays relevant to disease. You will learn to produce disease-aggregation prone-protein including TDP-43 aggregates recombinantly, as well as isolate them directly from the brains of animal models and human donors. The seeding capacity of TDP-43 aggregates will be studied comprehensively in vitro, in cells, and in animal models.
About the Switch laboratory
The SWITCH Laboratory, part of the KUL Department of Cellular and Molecular Medicine and the VIB-KU Leuven Center for Brain & Disease Research, is one of the leading laboratories in human disease-related protein aggregation. It is an interdisciplinary workplace, bringing together about twenty-five researchers from different backgrounds, such as bioinformatics, biophysics, and cell biology. SWITCH has a unique platform of technologies for studying protein aggregation, including biophysical, ultrastructural, and cell biological instrumentations.
For a primer on who we are, what we do, and our latest publications, see http://www.switchlab.org/
About the Da Cruz Laboratory of Neurophysiology in Neurodegenerative Disorders
The Da Cruz Laboratory, part of the KUL Department of Neurosciences and the VIB-KU Leuven Center for Brain & Disease Research investigates disease mechanisms for therapy development to ultimately treat ALS and FTD. We focus on the role of local axonal translation and liquid-liquid phase separation/aggregation as well as the spreading of disease-associated RNA binding proteins TDP-43 and FUS in axonal/synaptic maintenance and loss. To unravel novel disease targets, we combine sophisticated mouse models, and human stem cell and genome editing approach to model ALS and FTD, together with state-of-the-art spatial omics technologies and high-resolution microscopy. We are a stimulating international team bringing together postdocs, Ph.D. and Master students, and lab technicians.
See https://dacruzlab.sites.vib.be/en to learn more about us.
What we are looking for
- You hold an M.Sc. or M.D. degree, with minimally distinction grades or higher.
- You meet the English language criteria of KU Leuven
- You have a strong interest in protein aggregation and neurodegenerative diseases
- Highly motivated, enthusiastic, critical, and creative
- The ability to work in a multidisciplinary team is a must
- Prior experience with high content analysis is a plus
- You are willing to work with patient tissue and conduct animal experiments (Felasa B certificate is a plus)
- A versatile and challenging job
- A vibrant, world-class research environment operating at an international level
- Access to the resources of two host institutions:
- VIB an excellence-based research institute
KU Leuven , one of Europe’s leading research universities
- State-of-the-art research facilities .
- A dedicated training program to broaden your expertise and enhance your skillset
Starting Date: as soon as possible
How to apply?
For more information contact firstname.lastname@example.org . Please complete the online application procedure and include a detailed CV including a list of publications, a motivation letter, and the contact information of three referees.
Discover our Center and visit the website , read our brochure , or look around during a virtual tour
Follow us on social media: Facebook and Twitter
Ph D Scholarship In The Biological Roles Of Protein Aggregate Stability Dtu Bioengineering, Technical University of Denmark, Denmark, 1 day ago
The research group of Prof. Alexander K. Buell at the department of Biotechnology and Biomedicine at the Technical University of Denmark (DTU Bioengineering) is looking to recruit a PhD student, t...
Early Stage Researcher (Ph D Student Candidate) Marie Curie Innovative Training Network (Itn) Train Ck Dis:Multidisciplinary Training In Chronic Kidney Disease: From Genetic Modifiers To Drug Discovery , University of Zurich, Switzerland, 2 days ago
Institute of Physiology, Mechanisms of Inherited Kidney Disorders Early stage Researcher (PhD student candidate) - Marie Curie Innovative Training Network (ITN) TrainCKDis:Multidisciplinary Trainin...
Ph D Position Structural Biology Of Neurodegeneration, Delft University of Technology, Netherlands, 4 days ago
How do we remember and why do we forget? What are the molecular mechanisms at play during neurodegenerative diseases, such as Alzheimer, FTD and Parkinson? A 4-year position is available for a PhD...
Ph D Position In Systems Biology (1.0 Fte), University of Groningen, Netherlands, about 20 hours ago
Eukaryotic cell growth is tightly regulated by a set of conserved signaling pathways that monitor the extracellular and intracellular environment. In dividing cells, the activity of these pathways...
Ph D Position Structural Biology Of Neurodegeneration, Delft University of Technology (TU Delft), Netherlands, 14 days ago
PhD position Structural Biology of Neurodegeneration PhD position Structural Biology of Neurodegeneration Published Deadline Location yesterday 15 Apr Delft How do we remember and why do we forget...
Ph D Position (Ageing & Disease; Rna Biology): Finding A Therapy For Huntington’s Disease (M/F/D)Ph D Individual Supervisor, DAAD, Germany, 1 day ago
Description Thinking of doing your PhD in the Life Sciences? The International PhD Programme (IPP) on Gene Regulation, Epigenetics & Genome Stability is offering talented, young scientists the ch...