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barcode→(x,y) maps for Pixel‑seq; error correction; join gene/protein counts to spatial coordinates; QA of mapping rates. Segmentation & QC – 20% Apply/benchmark nuclei or whole‑cell segmentation (e.g
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: ● Strong foundation in mass spectrometry-based proteomics, including data acquisition strategies such as DDA and DIA, protein identification, and quantitative workflows across diverse sample types including
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material while creating genetic diversity in each generation. Our lab introduced the use of single-molecule tracking microscopy in live meiotic nuclei in C. elegans, revealing how protein diffusion, binding
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improve procedures currently used in the lab, including NGS, viral load and viral detection, gene expression, cell culture, and protein expression for binding antibody and general immunology testing. Draft
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brain regions; tissue fixation, embedding, and cryosectioning; brain tissue antigen retrieval, histochemical and immunofluorescent identification of protein expression; embryonic and postnatal mouse brain
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Knowledge, Skills and Abilities: ● Working knowledge of common molecular biology techniques such as qPCR, IHC, protein extraction and assessment, sterile cell culture, cloning, etc ● Ability
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(PCR, sub-cloning, recombinant protein expression and purification). Operation and maintenance of laboratory equipment including minor repair and liaising with external maintenance companies. Training
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, DNA-protein interaction, and cell biologic studies. Undertake bioinformatics analyses of imaging, genomics, transcriptomics and proteomics data, as well as statistical analyses of biological data and
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research and train the next generation of scientists. Dr. Eric Poeschla’s laboratory investigates cellular proteins involved in viral replication cycles (HIV-1, other positive strand RNA viruses). Current
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kinase in the nucleus Supervisor: Ruaidhrí Carmody Project description: The TPL-2 kinase is a key activation of mitogen activated protein kinases (MAPK) in innate immune cells and is required