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Field
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. - Molecular biology responsibilities include, DNA/RNA extraction and quality control (Nanodrop, Bioanalyzer/TapeStation), PCR / qPCR (including RT-qPCR for gene expression), Western blotting (including co-IP
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cell biology methods. The work will include tissue preparation and cell isolation, cloning, PCR, flow cytometry, ELISA, cell culture, and the handling of primary human and murine samples. The position
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Proficiency in structural biology and preferably knowledge in bioinformatics and/or patch clamp measurements and/or data-driven protein design Experience with basic molecular biology techniques (PCR, cloning
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | about 1 month ago
(e.g., -lactamases and carbapenemases, efflux, porin loss) and mobile genetic elements. Molecular biology experience (PCR/qPCR, cloning, plasmid isolation and characterization). Experience with
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. ● Experience with mouse models. ● Experience with cell and molecular biology techniques including PCR, western blotting, flow cytometry, cell culture and immunohistochemistry assays. Position Summary
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. ● Experience with mouse models. ● Experience with cell and molecular biology techniques including PCR, western blotting, flow cytometry, cell culture and immunohistochemistry assays. Position Summary
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Analysis - Analyze DNA repair outcomes using PCR, sequencing, genotyping, HRMA, Sanger-based editing analysis, nanopore sequencing, and related molecular approaches. -Evaluate homing, end-joining, SSA, indel
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reviewing standard operating procedures. The role includes processing and inventorying human tissue samples and using specialist laboratory equipment such as tissue culture hoods, PCR machines, microscopes
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, western blotting, qRT-PCR, IHC/immunofluorescence, FACS, bulk and sc-RNA-sequencing analysis is essential. You will have excellent communication skills, including the ability to write for publication
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. Experience of viability assays, western blotting, qRT-PCR, IHC/immunofluorescence, RNA-binding assays, R-loop assays, northern blotting, radiobiology, RNA sequencing analysis and FACS is essential. You will