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Field
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, perform Western blotting assays and quantitative RT-PCR assays. Independently monitor and carry out oversight of animal lines including transgenic mice colony maintenance, employing gel electrophoresis and
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a stroke or dementia lab, doing stroke surgeries (MCAO model), other vascular surgeries, molecular biology experiments (western blotting, RT-PCR, RNA-DNA isolation, immunofluorescence, histology, etc
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tissue, and Western blotting and biochemical assays on brain tissue lysates. S/he should also be able to operate standard laboratory equipment such as centrifuges, culture hoods, incubators, PCR machines
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. Standard molecular biology techniques such as PCR and cloning. About the lab and St. Jude: St. Jude Children's Research Hospital is a world-class research institution dedicated to pediatric cancer and other
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editing, PCR, Sanger sequencing Track-record of first-author publications in reputable peer-reviewed journals Demonstrated self-initiative and motivation, ability to collaborate, organize parallel
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background in molecular genetics. The candidates should be very familiar with the techniques, including Cell culture, Gene transfection, RNA extract, RT-PCR; Real-time quantitative PCR, DNA cloning, Plasmid
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including but not limited to immune cell culture assays, flow cytometry, Western blotting, Q-PCR, library creation and RNA and DNA sequencing Experience working with human samples is preferred Strong writing
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | about 2 months ago
Summary Perform immunological assays including T cell Elispots, T cell Proliferation,FACS analysis, FLOW sorting, Magnetic bead separation of immune cells, chlamydia mouse model work, tissue culture, PCR
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protein isolation from tissue and cultured cells, real-time PCR, Western blotting, molecular biology, histology, immunocytochemistry, and fluorescence microscopy (confocal/multiphoton). The candidate should