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of gene targeted and transgenic mice with skills to include microinjection of zygotes with CRISPR-Cas9 reagents, ES cell blastocyst injection, ES cell morulae aggregation, pronuclear injection of DNA
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knowledge regarding the immunologic functions of distinct cell types in primary brain tumors, to capture novel immunologic consequences of distinct glioma genotypes and to build novel oncoimmunologic
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focusing on myocardial biology and heart failure pathophysiology. Job functions will involve cell culture techniques including work with induced pluripotent stem cells (iPSCs) and iPSC-derived
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. The position requires extensive knowledge of and experience with Becton Dickinson cell sorters, advanced knowledge of biosafety practices and procedures, and at least 1 year of direct experience operating in
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mouse crosses, performing DNA extractions, PCRs, running gels, genotyping, Western blotting, and cell culture. The candidate would be expected to maintain and keep accurate laboratory records and
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funding. Qualifications: Bachelor of Science and 0 - 1 year of experience or equivalent combination of education and experience is required. Experience in surgical techniques and animal experiments, cell
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cloning and cell culture. Molecular cloning duties include DNA digests, ligations, plasmid preparations and sequencing analyses. Cell culture duties include expansion/freezing of primary human T cells and
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vitro/ex vivo evaluation of RNA protein expression in cell culture models and primary cells from blood or tissues of humans, mice, or non-human primates. As such, they will maintain cell lines and primary
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inventory organization and maintenance -- Perform additional duties as assigned Qualifications: Bachelor of Science and 0 to 1 year of experience or equivalent combination of education and experience is
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management, safety, organization, and hazardous goods shipping. Job Description Research Specialist C will conduct experiments and provide technical support for single-cell projects within the Shaffer lab