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Generation Sequencing (NGS, also known as Massively Parallel Sequencing) has revolutionized molecular genetic research and clinical diagnostics over the past decade. Appropriately scaled, this technology can
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RAP opportunity at National Institute of Standards and Technology NIST Development of Technologies to Enable Single Molecule Protein Sequencing Location Material Measurement Laboratory
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RAP opportunity at National Institute of Standards and Technology NIST Design, Characterization, and Modeling of Sequence Controlled Polymers Location Material Measurement Laboratory, Materials
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that align DNA sequencing reads to the reference, but long and linked read sequencing technologies are now enabling haplotype-separated “de novo assembly” of human germline and cancer genomes. These methods
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(STR) markers via capillary electrophoresis and next-generation sequencing, as well as mitochondrial genome sequencing. Additionally, NIST focuses on the deconvolution of DNA mixtures, rapid PCR methods
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, environmental, and microbiome analyses. Metagenomic sequencing holds the promise of enabling culture-free, non-targeted identification and characterization of a mixed population of bacteria, viruses, archaea, etc
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commercial assays. Common methods of characterization include copy number determination by quantitative PCR and digital PCR, as well as sequencing by Sanger and next-generation platforms. Projects focus
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benchmarks for genome sequencing methods [1]. In addition, these cell lines have been used as a well-characterized background DNA in over 50 commercial products. These cell lines, as well as induced
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microbiome measurements. Research topics include (1) quantitative descriptions of the uncertainty and bias present in current and emerging sequencing and metabolomics measurement capabilities; (2) round-robin
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) the dependence of peptide signaling sequence structure in cell adhesion studies, important for tissue engineering and implants. In a new class of experiments, we are developing ultraviolet-infrared doubly-resonant