Synthetic Biology – Enzyme Evolution and Design Postdoctoral Scholar

Updated: 4 months ago
Location: Berkeley, CALIFORNIA

Berkeley Lab’s Environmental Genomics & Systems Biology Division has an opening for a Synthetic Biology – Enzyme Evolution and Design Postdoctoral scholar.  The Post-Doctoral Fellow working in the Yoshikuni Group is responsible for developing a function database of all aminotransferases found in several energy crops and to build predictive models for the function of these aminotransferases. Successful delivery of this project allows to improve our understanding of plant NFMs and may enable improving sustainable growth of crops. Additionally, we can potentially provide synthetic biology communities a collection of aminotransferases for production of renewable chemicals and medicines.

What You Will Do:

  • Design and build expression constructs

  • Site-directed satulation mutagenesis

  • Protein expression and purification

  • Biochemical characterization of enzyme function (e.g. specific activity, steady-state kinetics

  • Homology protein structural modeling

  • Pfam analysis

  • Multiple sequence alignment

  • Tree/sequence similarity network construction and analysis

  • Co-evolution analysis

  • Build a predictive model

  • RNAseq analysis

What is Required: 

  • Ph.D. degree in Biochemistry

  • Ability to work in a dynamic and diverse team environment

  • Strong written and oral communication skills

Additional Desired Qualifications:

  • Experience in molecular biology techniques (molecular cloning, DNA/RNA extraction, PCR, gel electrophoresis).

  • Experience in biochemical characterization of enzymes (protein purification, specific activity, steady-state kinetics).

  • Sequence-based enzyme analysis (e.g., BLAST, pfam search, Multiple Sequence Alignment, tree construction, visualization)

  • Structure-based enzyme analysis (e.g., pymol, ROSETTA)

The posting shall remain open until the position is filled .


  • This is a full-time-year postdoctoral appointment with the possibility of renewal based upon satisfactory job performance, continuing the availability of funds and ongoing operational needs. You must have less than 2 years of paid postdoctoral experience. Salary for Postdoctoral positions depends on years of experience post-degree.

  • Full-time, M-F, exempt (monthly paid) from overtime pay.

  • This position is represented by a union for collective bargaining purposes.

  • Salary will be predetermined based on postdoctoral step rates.

  • This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.

  • Work will be primarily performed at DOE Joint Genome Institute (JGI) — 1 Cyclotron Road, Berkeley, CA.

Berkeley Lab ( LBNL ) addresses the world’s most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab’s scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy’s Office of Science.

Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4.  Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."

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