Staff Research Associate

Updated: 20 days ago
Location: San Francisco, CALIFORNIA

MED-CORE-PULM

Full Time

77621BR


Job Summary

The incumbent in this position will be involved in research centered on the epithelial biology of lung repair and regeneration after injury and during aging. Our laboratory is also engaged in developing drugs or molecular tools to favorably regulate the repair process and prevent fibrosis.  Current projects in the lab focus on understanding the impact of age on alveolar repair in normal and diseased human lungs (Cohen et al. BioRxiv 2023, Jourdan Le Saux et al. Am J Res Cell Mol Bio 2024).   The position will involve acquiring skills in culturing lung slices for in vitro assays, isolating primary cells from human lungs and their subsequent analysis by flow cytometry. The incumbent in this position will also manage and accurately maintain mouse strains, which including mouse genotyping and testing transgenic mouse lines.  Collectively, this position should provide a solid foundation for subsequent training at a graduate school level.

This position will be working directly under Dr. Hal Chapman at the Chapman Lab the Parnassus campus University of California San Francisco.

Specific duties include:

  • Isolation and culture of Type II cells from human lungs with varying conditions including preparation of co-cultured system such as organoids
  • Preparation of precision cut lung slides and culture with varying conditions
  • Isolation and preservation of mouse lungs for subsequent biochemical and histological assays.
  • Measurement of total lung collagen in frozen mouse lungs.
  • Isolation of PCLS, organoids, and whole lungs for OCT embedding and biochemistry
  • Isolation of RNA, DNA and protein from various cells and tissue
  • Sectioning of human and mouse lungs.
  • Immunofluorescence and X-gal staining of primary cells, cells lines, and lung sections.
  • Isolating genomic DNA and performing PCR analysis for mice genotyping.

The final salary and offer components are subject to additional approvals based on UC policy.

To see the salary range for this position (we recommend that you make a note of the job code and use that to look up): TCS Non-Academic Titles Search (https://tcs.ucop.edu/non-academic-titles)

Please note: An offer will take into consideration the experience of the final candidate AND the current salary level of individuals working at UCSF in a similar role.

For roles covered by a bargaining unit agreement, there will be specific rules about where a new hire would be placed on the range.

To learn more about the benefits of working at UCSF, including total compensation, please visit: https://ucnet.universityofcalifornia.edu/compensation-and-benefits/index.html


Department Description

The Pulmonary, Critical Care, Allergy and Sleep Medicine Division and the Zuckerberg San Francisco General (ZSFG) Lung Biology Center in the Department of Medicine (DOM) have a long history of providing outstanding clinical care, performing innovative research and training academic scientists and physicians. We currently have active, well-funded clinical, translational and basic research programs in lung development, international lung health, pulmonary immunology, genetics of pulmonary and allergic diseases, lung transplantation, microbial pathogenesis, airway diseases, acute lung injury, pulmonary fibrosis, pulmonary malignancies and pulmonary health disparities. Our large, National Institutes of Health (NIH) training grant has been continuously funded since 1966. Nearly a quarter of all academic pulmonologists in the United States have trained at UCSF. This combined unit includes: seven outpatient clinics, four inpatient services, a pulmonary function diagnostic center, 15 independent wet labs, four independent research service cores, six independent clinical research units, one specialty destination program, and the Nina Ireland Program in Lung Health focusing on philanthropic activities.


Required Qualifications

  • BA/BS in a related science and one or more years of recent, directly related work experience, utilizing the techniques or methods required by the position; and/or equivalent experience/training.
  • Knowledge of and experience in cell/molecular biology as well as mouse handling, including experience in cell culture, immunoprecipitation, and immunostaining.
  • Willingness and ability to work with small animals/mice
  • Computer skills at the level of using spreadsheet programs
  • Careful attention to detail. Maintain a detailed laboratory notebook of experiments done on a daily basis
  • Excellent organizational skills and multitasking
  • Experience handling mice
  • Strong written, verbal, data recording and organizational skills
  • Demonstrated proficiency in handling and working with mice, including anesthesia, ear tagging, intratracheal and peritoneal injections, and euthanasia
  • Computer skills at the level of using spreadsheet programs
  • Sound quantitative skills

Preferred Qualifications

  • Demonstrated proficiency in mammalian cell culture and primary cell isolation from animal tissue techniques including maintenance of cell lines, transfection techniques, isolation of RNA and DNA
  • Demonstrated proficiency in biochemical techniques such as ELISA, protein measurements, and collagen assays.
  • Familiarity with Flow Cytometry
  • Experience with PCR

About UCSF

The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. It is the only campus in the 10-campus UC system dedicated exclusively to the health sciences. We bring together the world’s leading experts in nearly every area of health. We are home to five Nobel laureates who have advanced the understanding of cancer, neurodegenerative diseases, aging and stem cells.


Pride Values

UCSF is a diverse community made of people with many skills and talents. We seek candidates whose work experience or community service has prepared them to contribute to our commitment to professionalism, respect, integrity, diversity and excellence – also known as our PRIDE values.

In addition to our PRIDE values, UCSF is committed to equity – both in how we deliver care as well as our workforce. We are committed to building a broadly diverse community, nurturing a culture that is welcoming and supportive, and engaging diverse ideas for the provision of culturally competent education, discovery, and patient care. Additional information about UCSF is available at diversity.ucsf.edu

Join us to find a rewarding career contributing to improving healthcare worldwide.


Equal Employment Opportunity

The University of California San Francisco is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, protected veteran or disabled status, or genetic information.


Job Code and Payroll Title

009617 SRA 2 NEX


Job Category

Research and Scientific


Bargaining Unit

University Professional Technical Employees – Research Support Professionals Unit (UPTE-RX)


Location

Parnassus Heights (SF)


Additional Shift Details

Monday-friday 8hr/day



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