Under the direction of the Principal Investigator along with supervision and coordination of junior faculty, Assistant/Associate Researchers, and postdoctoral fellows, we explore and validate gene regulation and function in hepatocytes after identifying important candidate in transcriptomics and lipidomics research from humans with nonalcoholic fatty liver disease (NAFLD) with and without hepatocellular carcinoma. We also examine the molecular factors associated with NAFLD, obesity and other metabolic diseases.
In collaboration with laboratory personnel, you will perform basic experiments using cell culture models (including primary cell cultures), cultured cell extracts, cloning, stable cell line generation, and human samples to compile fundamental data, the necessary tools for strengthened analysis, theorizing, and understanding. Work with murine models and also undertake novel, independent research projects in collaboration with other lab members. General lab duties include overall lab maintenance, ordering, inventory assessments, and basic training and superintending of junior scientist, students, and interns or fellows.
UCLA is an Equal Opportunity/Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected Veteran status.
• Over 10 years of experience in the laboratory
• Preparation of appropriate cell culture media using proper sterilization technique
• Both DNA and RNA extraction, purification, amplification via PCR/RT-PCR
• CRISPR-Cas 9 gene editing and DNA site-directed mutagenesis
• Recombinant adenovirus and adeno associated virus (AAV) construction and viral particle packaging and titer testing
• Agarose and acrylamide gel preparation and electrophoresis
• Primer construction and selection
• DNA, RNA, and protein spectral quantification
• Proper animal care in observance of AAALAC, IACUC, NIH regulations
• Animal injection, feeding, handling, dissection/harvesting, preserving desirable tissues for histological, protein, serum, and RNA analysis
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