Staff Research Associate II

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  JOB DUTIES
  

Under supervision of the Principal Investigator and the Postdoctoral Fellow, the Staff Research Associate II (SRA II) will provide basic technical support in research laboratory understanding the molecular and genetic basis of neurological diseases will provide technical support in research focused on the molecular and genetic basis of developmental and neuro-psychiatric disorders. This includes bench work involving molecular analysis of RNA/DNA, preparation of single-cell libraries, immunohistochemistry, and genomics. The vast majority of work will be tissue culture (cell culture maintenance, generating stable cells lines, primary neuronal cultures, stem cells including iPSCs); transfection in cellular studies; molecular cloning; RNA, DNA and protein extraction and analysis (PCR, RT-PCR, western blot); tissue collection from mice; immunocytochemistry and immunohistochemistry handling human tissue and in vitro organoids for genomic analysis including, but not limited to, single-cell transcriptomics and epigenomics, immunohistochemistry, and In Situ RNA hybridization. Additional work will include basic bioinformatics, tissue dissection, RNA/DNA extraction, western blotting, and qPCR.. Furthermore, the SRA II will be involved in transformation and generating DNA plasmids; performing PCR and gel electrophoresis; will be involved in generating spatial transcriptomics, immunocytochemistry and immunohistochemistry, preparing buffers, solutions, and chemicals for lab experiments; basic data analysis and interpretation; maintaining database of experimental results; assisting with figures for grant applications and publications; providing technical assistance in the lab, including assisting other members of the lab; and assisting with training of lab assistants and students. Salary Range: $28.04 – $45.11 Hourly

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  JOB QUALIFICATIONS
  

Required:. Extensive experience in Tissue culture and general knowledge of molecular biology, genetics, cell biology, human genetics, and/or neuroscience theories and principles. Knowledge of standard laboratory equipment and supplies. Good communication skills and ability to work well with multiple individuals. Ability to maintain an organized working area. Ability to keep accurate and organized lab records. Ability to work on several projects simultaneously and to switch focus as needed. Knowledge of chemistry to prepare solutions for lab experiments. Knowledge of laboratory safety procedures for handling chemical, biohazard, and medical waste. Knowledge of basic molecular biology/cell biology/genetic techniques. Working knowledge and ability in the use of computers (PC and MAC operating systems). Skill in using various computer software (e.g., Microsoft Office, Adobe products, etc.) Excellent attention to detail, for accurate data entry and analysis. Good organization and time management skills. Always punctual. Ability to learn new laboratory techniques and new lab equipment, as needed. General knowledge of University procedures and compliance with Environmental Health and Safety guidelines. Preferred: B.S. degree or equivalent in Molecular Biology, Microbiology, Cell Biology, Biochemistry or related field. Willingness to work occasional overtime in order to meet deadlines related to grant applications and publications.