Classified Title: Research Technologist
Working Title: Research Technologist
Starting Salary Range: $14.00-$19.25
Employee group: Full-Time
Schedule: M-F; 8:30am-5:00pm
Exempt Status: Non-Exempt
Location: 33-MD:Johns Hopkins Bayview
Department name: 10002817-SOM DOM Pulmonary
Personnel area: School of Medicine
The laboratory studies the basic mechanisms regulating myocardial angiogenesis and right ventricular remodeling in pulmonary hypertension. The Research Technologist will perform and analyze an array of experiments using isolated endothelial cells in culture and tissue samples obtained from animal models of pulmonary hypertension and patients with pulmonary hypertension. Requires a proficient Research Technologist with a sophisticated understanding of techniques and a solid foundation in the underlying science gained from education and personal experience. Participates in the design of experiments, analyzing data, presentation at lab meetings, and may contribute to manuscripts. Will provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.
Essential Job Functions
- Cell culture and maintenance. Must be knowledgeable of good tissue culture techniques and basic principles of light microscopy; previous work with endothelial cells is a bonus
- Must be proficient in basic molecular biology techniques, including protein/DNA/RNA extraction and quantification from cells and tissues, Western blotting, ELISA, quantitative PCR
- Immunostaining and immunofluorescence microscopy. Previous exposure to basic image analysis programs (Image J) preferred.
- Preparation and staining of cells for flow cytometry analysis
- Analyzes data, formulates conclusions, and creates appropriate statistical presentations, including graphs and charts for the purpose of reporting research/laboratory results and quality control. Attend and present data in weekly lab meetings.
- Conduct literature searches; work with PI and other lab members on manuscript preparation
- Independent troubleshooting of technical and experimental issues
- Basic lab management responsibilities, including preparing stock solutions, buffers and media, upholding safety regulations, maintaining records, and managing supplies
Minimum qualifications (mandatory):
Requires bachelor’s degree in biology, chemistry, or another related field. Six months related experience.
Some related post-undergraduate work experience.
Experience in a comparable laboratory performing relevant techniques, coupled with a survey knowledge of biological sciences, chemistry, or physics
Special knowledge, skills, and abilities:
Exchanges routine and special information in an appropriate manner requiring good oral and written communication skills.
- Work produced is subject to precise measures of quantity and quality
- Work environment may include areas of unpleasant extremes of cold or heat
- Biohazardous conditions such as the risk of fumes, toxic or caustic chemicals, and human cells or tissues are present in this work environment, which mandate attention to safety considerations.
- Near vision to see objects clearly within 20 inches
- Sharp focus to adjust vision when doing close work that changes in distance from eyes
- Full spectrum vision to identify and distinguish color
- Handling by seizing, hold, grasping, turning, or otherwise working with the hand or hands but without finger dexterity
- Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s); for example, using a keyboard
- Communication skills using the spoken word
- Occasionally lifting and carrying objects weighing 10 pounds or less
- Occasionally pushing and/or pulling objects weighing 30 pounds or more
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at firstname.lastname@example.org . For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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