Research Technologist I

Position Description:
Research Technologist I

Purpose A Research Technologist I position is immediately available in the E lab at the Neuroscience Research Center at the Medical College of Wisconsin. The lab investigates non-neural mechanisms of neurodegeneration in normal aging, as well as genetic underpinnings of Alzheimer’s disease-related neurodegeneration, primarily using C. elegans (roundworm) as a model system. The lab is seeking a motivated Research Technologist to provide support for lab research through ordering and maintaining supplies, preparing reagents/media, maintaining the biological material database/libraries, and managing general lab maintenance. Under the direction and supervision of the principal investigator, the Research Technologist is also expected to execute experiments/assays to generate data for research projects/publications/grant applications. While experience in molecular biology, molecular genetics, microscopy, and/or working with C. elegans is welcome, hands-on training will be available for all required tasks. Demonstrated proficiency in basic biology/chemistry lab techniques (weighing, pipetting, making solutions/buffers, dilutions, centrifugation, etc.) is required. Individuals with experience in molecular biology (standard PCR, qPCR, plasmid design and construction) are encouraged to apply.

Primary Functions
• Prepare sterile buffers, media, reagents, nematode culture (nutrient agar) plates and competent cells for transformation etc.
• Execute assigned duties for ordering and maintaining the supply of inventories.
• Manage and organize the C. elegans strain/plasmid/primers/RNAi library collection in the lab.
• Perform and assist in assigned assays, including but not limited to PCR, qPCR, worm picking, worm genotyping, molecular cloning, microinjection to generate transgenic animals, in vivo imaging using widefield and confocal fluorescence microscopy, and quantification of neurodegeneration phenotypes.
• Record and compile accurate records and communicate summaries of experimental results.
• Assist with the evaluation, interpretation and assessment of experimental data and quality control of assays.
• Attend lab meetings with principal investigator, graduate students, research staff, participate in discussion, and present data as needed.
• Maintain lab records and uphold safety regulations.
• Perform routine maintenance on specialized equipment used in the lab. Communicate with vendors for troubleshooting in case of malfunctioning.
• Oversee daily lab operation in the absence of the principal investigator.
• Will have opportunities to contribute to publications.

Knowledge – Skills – Abilities
Knowledge of biology, chemistry, mathematics, documentation, and records management.
Data utilization, complex problem solving, critical thinking, resource management, and communication/writing skills.

Preferred Schedule:
8A-5P M-F

Position Requirements:
Specifications
Appropriate experience may be substituted for education on an equivalent basis
Minimum Required Education: Bachelor’s degree in Biology or related field
Minimum Required Experience: At least 2 consecutive months of hands-on experience in a biology lab (wet lab)
Preferred Education: Preferred Experience: Molecular biology and/or fluorescence microscopy are a plus.
Field: Cell and molecular biology, neurobiology, genetics
Certification:

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The Medical College of Wisconsin (MCW) is an Equal Opportunity Employer. We are committed to fostering a diverse community of outstanding faculty, staff, and students, as well as ensuring equal educational opportunity, employment, and access to services, programs, and activities, without regard to an individual's race, color, national origin, religion, age, disability, sex, gender identity/expression, sexual orientation, marital status, pregnancy, predisposing genetic characteristic, or military status. Employees, students, applicants or other members of the MCW community (including but not limited to vendors, visitors, and guests) may not be subjected to harassment that is prohibited by law or treated adversely or retaliated against based upon a protected characteristic.

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