Research Technician in Molecular Biology

University of North Carolina, United States

Updated: about 1 year ago
Location: Chapel Hill, NORTH CAROLINA
Job Type: PartTime
Deadline: 28 Feb 2023

Posting Information
Posting Information


Department Applied Physical Sciences - 318100
Career Area Laboratory Management and Services
Posting Open Date 02/10/2023
Application Deadline 02/28/2023
Position Type Temporary Staff (SHRA)
Position Title Research Technician in Molecular Biology
Position Number 20053514
Vacancy ID S020915
Full-time/Part-time Part-Time Temporary
Hours per week 20
Work Schedule
Flexible
Position Location North Carolina, US
Hiring Range $15.00 - $20.00 per hour
Proposed Start Date
Estimated Duration of Appointment 6 months not to exceed 11 months

Position Information


Primary Purpose of Organizational Unit
Launched in 2013, the Department of Applied Physical Sciences (APS) is the first new science department in the College of Arts and Sciences in nearly 40 years. Including the Chapel Hill Analytical and Nanofabrication Laboratory (CHANL) and the graduate program in material sciences, Applied Physical Sciences has united numerous scientific entities on campus to further its mission of finding interdisciplinary solutions to real-world problems. Its newest endeavor, BeAM (Be A Maker), is a university-wide initiative that incorporates Making into research, education, and entrepreneurship. BeAM is comprised of several spaces, including a makerspace in Hanes Art Center, Carmichael Residence Hall, and a 3,400 square foot Makerspace in Murray Hall, all of which serve the entire UNC community.
Position Summary
The Freeman Lab at the department of Applied Physical Sciences at UNC-Chapel Hill is an interdisciplinary space dedicated to the study of basic and translational science required to understand cell-extra cellular matrix interactions for tissue engineering and regenerative medicine.
The Freeman lab seeks a research technician/assistant responsible for the planning, development, and implementation of molecular biology experimental procedures, performing laboratory techniques, and collecting, documenting, evaluating, interpreting, and analyzing data to identify trends and appropriate conclusions.
The Research Assistant will perform molecular biology techniques; transform and culture bacteria for protein production; perform routine culture, transfection, maintenance and assay of stem cells and human cell lines, perform experiments involving PCR , preparation of DNA and RNA , protein purification and western blot analysis. The technician will also assist with the day-to-day management of the bio-related research laboratory operations including supervising students and ensuring compliance with chemical and biosafety regulations. Attendance at regularly scheduled lab meetings is expected.
The Research Assistant must be able to read and understand the scientific literature and execute experiments as outlined in the protocols. High level of responsibility, and independence are required for this position. The candidate for the Research Assistant should have a solid background in molecular biology techniques and must master the aseptic technique for tissue culture purposes, must be extremely organized and detail oriented, capable of working on multiple projects simultaneously and maintain detailed notes on each experiment.
Responsibilities, Tasks and Outcomes:
Culturing stem cells and human cell-lines.
Perform cell manipulation procedures such as cell enrichment, cell passage, cryopreservation and cell product thaw/wash.
Performs calculations including for reagents dilutions and cell concentrations.
Adhere to institutional and regulatory guidelines when handling, recording, and collecting data and samples.
Perform cellular and molecular assays, flow cytometry, western blot analysis and fluorescent imaging.
Minimum Education and Experience Requirements

Bachelor’s degree in a discipline related to the area of assignment; or equivalent combination of training and experience. All degrees must be received from appropriately accredited institutions.
Required Qualifications, Competencies, and Experience
Demonstrable evidence of successfully implementing molecular biology techniques in a research environment. Proficient with molecular biology bench techniques (PCR, gel electrophoresis, plasmid construction, transformation, CRISPR -Cas9, etc.). Familiarity with microplate readers, coulter counters, cell enumeration techniques, colorimetric / fluorescent assays and related instrumentation to evaluate targeted modifications. Independent troubleshooting and experimentation. Ability to learn quickly in a fast-paced environment. Ability to interface with external laboratories and consultants. Organized and possess excellent attention to detail; creative, flexible, proactive, punctual, and persistent; a team player; able to Self-Manage; comfortable with a demanding fast-paced work environment and able to excel in a growing environment. Understanding of basic laboratory techniques.
Preferred Qualifications, Competencies, and Experience
Experience with cellular and molecular assays, flow cytometry, western blot quantitative real-time PCR (qPCR) and reverse transcription q PCR (qRT-PCR), DNA and RNA sample preparation, Immunofluorescence assays is a plus.
Proficient with bioinformatics tools for primer design, construct design, and sequence analysis is a plus.
Special Physical/Mental Requirements
Campus Security Authority Responsibilities

Not Applicable.
Special Instructions
Quick Link https://unc.peopleadmin.com/postings/250105
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Posting Contact Information


Office of Human Resources Contact Information

If you experience any problems accessing the system or have questions about the application process, please contact the Office of Human Resources at (919) 843-2300 or send an email to [email protected]

Please note: The Office of Human Resources will not be able to provide specific updates regarding position or application status.
Equal Opportunity Employer Statement

The University of North Carolina at Chapel Hill is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to age, color, disability, gender, gender expression, gender identity, genetic information, race, national origin, religion, sex, sexual orientation, or status as a protected veteran.

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