Caltech is a world-renowned science and engineering institute that marshals some of the world's brightest minds and most innovative tools to address fundamental scientific questions. We thrive on finding and cultivating talented people who are passionate about what they do. Join us and be a part of the diverse Caltech community.
Job Summary
We are seeking a highly motivated and dependable individual with experience in molecular biology to join our integrative computational and experimental team in characterizing nuclear structure and architecture as it influences gene expression and functional outcomes at the cellular level, in many instances focusing on the functions and mechanisms of lncRNAs. Specifically, this person will help to optimize methods and generate data for understanding interactions between RNA, DNA, and proteins in mammalian cells. This work will include experiments to characterize RNA-protein interactions and DNA-protein interactions involving cell culture, cell line engineering using CRISPR methods, cloning tasks, library generation for high-throughput sequencing, and mass spectrometry.
The candidate will work closely with graduate students, post-doctoral fellows, and staff members in the lab to apply various technologies in specific biological applications. The candidate will adapt easily to changing needs and priorities of a fast-paced research environment, facilitate project completion, address technical issues as they arise, and will summarize and present results to the research group. Training in established techniques will be provided, but the work will require significant independence of day-to-day operations to achieve the overall goals of the project.
Essential Job Duties
With close guidance from senior members of the lab and in accordance with guidelines set forth for a particular task, the successful candidate will design and independently perform bench level experiments and small projects that support the overall research objective of an integrative computational and experimental lab in a fast paced and dynamic environment
Job tasks will include, but are not limited to, maintaining mouse embryonic stem cells including media and reagent preparation, cloning expression constructs and Cas9/CRISPR-based targeting constructs followed by transfecting validated plasmids into mouse embryonic stem cell lines; screening cell lines to identify successfully targeted clones; measuring functions of lines derived from these transfections. Functional assays will include, but are not limited to, RNA-Seq, ChIP-Seq, RNA Antisense Purification, DNA and RNA fluorescence in situ hybridization, flow cytometry, and RNA immunoprecipitation. In addition, techniques including, but not limited to, qPCR, immunostaining, fluorescent microscopy, and functional readouts will be required for quality control assessment.
The candidate will interact directly with senior members of the research team and lab head to ensure proper experimental design, analysis, and technical concerns related to the project. The candidate will adapt easily to changing needs and priorities, facilitate project completion, provide lab support and organizational needs, address technical problems, and summarize and present results. Training will be provided as needed.
Basic Qualifications
- Bachelor’s degree in biology or related field required.
- Experience in a research laboratory (not including a class setting)
- Solid technical knowledge of molecular cloning, cell biology techniques, and mammalian tissue culture
- Experience related to mammalian tissue culture.
- Familiarity and hands-on experience with molecular biological methods and the operation of standard lab equipment required.
- Excellent organization, time management, and project management skills
- Ability to record and report results with clarity.
- Must be detail-oriented and perform precise work in the laboratory.
- Persistence and willingness to troubleshoot technical challenges.
- Motivation to learn and master new methods and techniques.
- Excellent communication skills and the ability to interact professionally with all levels of staff and with external contacts in a fast-paced environment required.
- Ability to handle a variety of tasks simultaneously required.
- Must possess a high level of initiative and the ability to work with little supervision.
Preferred Qualifications
- Direct laboratory experience involving molecular cloning, synthetic biology, or cell biology a plus.
- Broad knowledge of cell biology, cloning, gene synthesis, and/or gene targeting approaches a strong plus.
- Basic knowledge of sequence analysis tools such as BLAST, primer3, UCSC genome browser, and other applications
- Basic familiarity with cellular transfection and/or gene perturbation
Required Documents
- Resume
- Cover Letter
Similar Positions
-
Scientific Research, California Institute of Technology, United States, 30 days ago
Details Posted: 29-Mar-24 Location: Pasadena, California Salary: Open Categories: Academic/Faculty Biological/Biomedical Sciences Internal Number: 5138826 Scientific Research Caltech Job Category:...
-
Research Associate I Ho Lab Center For Neural Science And Medicine, Cedars-Sinai Medical Center, United States, 9 days ago
Details Posted: 18-Apr-24 Location: Beverly Hills, California Salary: Open Categories: Academic/Faculty Internal Number: 1819 Job Description Principal Investigator, Dr. Ritchie Ho is seeking a Re...
-
Clinical Program Manager (Tuc Strength United), California State University, Northridge, United States, 3 days ago
About The University Corporation The University Corporation is a non-profit auxiliary corporation providing commercial and administrative services to California State University, Northridge. Our m...
-
Associate Director Of Programs (Tuc Strength United), California State University, Northridge, United States, 3 days ago
About the University Corporation The University Corporation is a non-profit auxiliary corporation providing commercial and administrative services to California State University, Northridge. Our m...
-
Research Scientist, Bioinformatics, Ismagilov Lab, California Institute of Technology, United States, 11 days ago
The Ismagilov Lab at the California Institute of Technology is looking for a Research Scientist, Bioinformatics to join a fast-paced ~20-person interdisciplinary laboratory team. The research agen...
-
Research Technician Associate, California Institute of Technology, United States, 18 days ago
Details Posted: 10-Apr-24 Location: Pasadena, California Salary: Open Categories: Academic/Faculty Biological/Biomedical Sciences Internal Number: 5167328 Research Technician-Associate Caltech Job...