RESEARCH TECHNICIAN II

Updated: 3 months ago
Location: Durham, NORTH CAROLINA

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.
Occ Summary

Perform a variety of complex technical duties inv olved in conductinglaboratory tests, experiments and determinations in s upport of DukeUniversity research operations.


Lab Background:
We are a high-energy, highly passionate translational neuroscience lab focused on understanding the role of synaptic plasticity in neurologic disease and how this can be directly modulated for disease modification (Harward et al., Nature 2016; Hedrick et al., Nature 2016; Krishnamurthy et al., Ann Neurol 2019). We are particularly interested in developing focused ultrasound as a new method for non-invasively modulating brain activity. Briefly, Focused Ultrasound has recently emerged as a ground-breaking new non-invasive, image-guided therapy to treat Essential Tremor (ET) and Parkinson’s Disease (PD). While it is used clinically to make small lesions in the brain circuitry responsible for both ET and PD, additional work has shown that when applied at lower intensity and frequency, it can be used to transiently and focally (with sub-millimeter precision) open the blood brain barrier, induce local immune responses, and activate excitatory neurons, inhibitory interneurons, astrocytes, and even microglia. Building on this work, we seek to harness the power of this new tool to optimize its therapeutic use for a wide variety of conditions including epilepsy, neuropsychiatric disease, movement disorders, chronic pain, and brain tumors. To accomplish these goals, we employ a wide range of experimental approaches including viral infections, mouse genetics, in vitro and in vivo preparations, electrophysiology, calcium imaging, optogenetics, and focused ultrasound. 
 
Job Description:
Fully funded research technician positions are immediately available. in the Department of Neurosurgery at the Duke University School of Medicine. The successful candidate will be an integral part of the research team, conducting independent experiments investigating focused ultrasound-based neuromodulation, presenting at weekly lab meetings and research conferences, and working with collaborators at Duke and beyond.
Requirements: Applicants should have at least a B.A. or B.S. degree, preferably in a biomedical science or related field. A highly motivated, independent candidate with a passion for translational neuroscience is preferred. Experience in neurobiology, electrophysiology, and in vivo mouse work is highly desirable but not required. 


Responsibilities will include:
•    Conduct biochemical assays including Elisa, PCR, Western blotting, and immunohistochemistry. Obtain, prepare, mount, and stain tissue for microscopic examination.
•    Prepare and maintain heterologous cell cultures (HEK and HeLa cells); prepare and maintain primary cultures of rat and mouse cortical neurons and glia; prepare and maintain organotypic hippocampal slice cultures of rat and mouse brains.
•    Manage the lab’s animal colonies in collaboration with DLAR and perform appropriate animal husbandry for rats and mice.
•    Perform stereotaxic surgery on mice for viral infection, electrical stimulation, focused ultrasound, and electrophysiological recordings.
•    Prepare culture media, chemicals, reagents, and solutions.
•    Assist the PI in maintaining lab animal, chemical, and safety protocols.
•    Maintain sufficient inventory of lab materials, supplies, and equipment for performance of duties; clean and maintain laboratory equipment; ensure all lab equipment, instruments, materials, and apparatuses are functional and meet safety guidelines
•    Place and track orders at the request of fellow lab members.
•    Keep up to date records of all lab work completed including experimental design, animal handling, and data analysis.
•    Perform other related duties incidental to the work described herein.

Minimum Qualifications
Education

Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.


Experience

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

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Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.



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