RESEARCH TECHNICIAN I

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Occupational Summary

Perform a variety of molecular biology techniques to generate recombinant antibodies for pathogen diagnostics.

Work Performed

Under general supervision of direct supervisors, the technician will: 1) perform selection procedures to obtain pathogen-specific antibody clones from M13 phage display libraries; 2) characterize antibody clones for specificity by performing immunoassays; 3) use molecular biology techniques such as PCR to clone antibody genes for recombinant expression; 4) perform cell culture and purify antibodies produced by expression cell lines; and 5) set up instruments, material and apparatus and operate laboratory equipment required for specific tests.

The technician will also perform a variety of complex technical duties involved in conducting physical, chemical, biological and other research laboratory tests, experiments and determinations to obtain data for research purposes; compile, analyze, document and draw tentative conclusions from experimental results; and confer with principal investigator to review work assignments and execute plans for research experiments.

The above statements describe the general nature and level of work being performed by individuals assigned to this classification. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.

 Required Qualifications at this Level

Education/Training:

Work requires a bachelor's degree in botany, biology, zoology or other science related scientific field.

Experience:

While no specific experience is required, prior experience with molecular biology (PCR, gel electrophoresis, restriction digests, plasmid preps), bacterial microbiology, M13 phage propagation techniques, or performance of routine immunologic assays would be preferred.

The position classification may be adjusted based on previous experience.

Skills:

N/A

Minimum Qualifications
Education

Work requires anAssociate's degree in botany, biology, zoology, psychology or other science related scientific field.

Experience

None required above education/training requirement OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

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Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.