Research Technician 2

Updated: 8 days ago
Location: Campus, ILLINOIS
Job Type: FullTime

Working under frequent supervision, the Research Technician 2 will conduct experiments related to diabetic retinopathy studies. This person will perform technical procedures and statistical analyses according to specific instructions/ protocols. Responsibilities include rodent modeling of diabetic retinopathy, retina cell isolation, photoreceptor cell isolation, and pathology analysis in retinal vasculature. The technician will also perform ELISA, flow cytometry, qPCR, and microscopy analyses of samples collected by supervising staff. The technician will contribute to the generating preliminary data and will be able to perform statistical analyses of data for grant submissions and publication manuscripts. Proficiency in core statistical methods and specialized skills in murine retina vasculature isolation are essential for success. This position will work with animals.


1. Perform technical procedures and experiments involving retina cell and vasculature isolation according to specific instructions/protocols. (15%) 
2. Record, tabulate, and calculate experimental data. Work with the supervisor and other investigating staff in the lab to develop statistical strategies and perform data analyses of various ongoing experiments. (15%)
3. Perform data mining of referenced topics from the supervisor and other investigating staff to assist in background, discussion, and reference sections of new grant proposals and manuscripts. (15%)  
4. Perform data management tasks, create analytic datasets that have been appropriately checked for errors, summarize data elements, and perform statistical analyses for grants and publications. (15%)  
5. Perform ELISA, flow cytometry, qPCR, and microscopy analyses of retina samples. (10%)
6. Prepare reagents for ELISA, flow cytometer, and Western immunoblot assays, perform housekeeping tasks and autoclaving, and assist in animal colony maintenance. (8%)
7. Purchase routine lab supplies, collect receipts, and monitor expenditures of the lab to follow budget. (6%)
8. Enter sequences into databases; may conduct analysis of datasets and perform statistical analyses for grants and publications. (6%)
9. Extract data for follow-up and data mining of referenced topics from the supervisor and investigating staff. (6%)


1. Learn new procedures as required. (2%)
2.  Perform other duties as assigned. (2%)


Department: Continuous daily contact with the Supervisor, and other investigating research staff of the supervisor's laboratory to discuss research, provide data and data analyses, and maintain workflow.
University: Occasional contact with other departmental staff in a lab meeting setting or during monthly research seminar series to share information. Monthly contact with collaborating faculty and laboratory staff from other departments in the university and of affiliate hospitals (i.e. UH and the Cleveland VA).
External: Limited contact with vendors to exchange information.
Students: Occasional contact with student employees to exchange information.


This position has no direct supervision of staff employees.


Experience: 1 to 2 years of related experience required.
Education: High school education required.


1. Has knowledge of commonly-used concepts, practices, and procedures within the vision science field. 
2. Relies on instructions and pre-established guidelines to perform the functions of the job. 
3. Ability to operate laboratory equipment and software to perform ELISA, flow cytometry, qPCR, and statistical analyses.
4. Ability to meet consistent attendance.
5. Ability to interact with colleagues and supervisors face to face.
6. Must demonstrate compliance with CWRU animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
7. Must demonstrate compassion for animals within CWRU facilities and dedication to the Animal Resource Center's mission. Must handle animals with care and respect at all times.
8. Previous experience working with animals preferred.
9. Must be able and willing to learn new techniques, procedures, processes, and computer gear to protect the health of the animals.


General laboratory environment. Will have exposure to hazards, such as, chemicals, bloodborne pathogens, and rodents. The proper Personal Protective Equipment (PPE) is supplied and will be worn daily as described during the initial onboarding safety training.
Case Western Reserve University's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals' appropriate Federal Animal Welfare Regulations, and the Public Health Service 'Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to medical school Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity.  Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Case Western Reserve University provides reasonable accommodations to applicants with disabilities.  Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation.  Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.


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