RESEARCH SCIENTIST/ENGINEER 2

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The University of Washington, Department of Laboratory Medicine and Pathology (DLMP) is a broad and dynamic department that strives for a consistent level of high achievement in all of its activities. The Department offers full diagnostic services in all areas of Laboratory Medicine and Pathology, is a top ranked research organization as measured by research awards from the National Institutes of Health, and offers an array of specialized educational training programs.

UW Medicine DLMP operations are based at the University of Washington Medical Center, Harborview Medical Center, South Lake Union, Northwest Hospital and the Seattle Cancer Care Alliance. DLMP faculty members also serve at Seattle Children's Hospital, the Veteran’s Administration Puget Sound Healthcare System, and the Fred Hutchinson Cancer Research Center. These collaborative efforts have generated innovative, translational studies in the basic mechanisms of disease.

The Rabinovitch/Sweetwyne Laboratory in the DLMP on the main campus at the University of Washington has an outstanding opportunity for a 1.0 FTE Research Scientist/Engineer 2. Working under the oversight of the principal investigator, the individual hired for this position will perform laboratory experiments, data analysis, and assist in preparing reports. This position is funded for at least 2 years with the potential for longer term support as funding permits.

The Research Scientist / Engineer 2 (RSE2) will report to, and work closely with, Dr. Sweetwyne, a senior co-investigator in the lab, but will be expected to work independently on research and development of projects focusing on the cellular mechanisms of renal damage and regeneration in the context of aging. The RSE2 will devise and troubleshoot protocols, generate and interpret data, and propose next steps for the development of the project. In this position the RSE2 will assume responsibility for coordinating work with outside collaborating research partners as well as collaborating with other members of the lab as needed. The RSE2 will be responsible for training a RSE1 and undergraduate research assistants in bench techniques and analysis routinely used in the laboratory as well as day-to-day oversight of lab tasks performed by trainees. The RSE2 will present their research progress at laboratory and departmental meetings and assist Dr. Sweetwyne in data analyses as relevant for the project and carry out project-specific literature searches and analyses, and manuscript and/or proposal writing.

RESPONSIBILITIES:
Specific research activities will include but will not be limited to:
• Primary cell culture (mouse, human) in 2D and 3D formats
• Interacting with human subject volunteers for sample collections
• FACS live cell sorting
• qRT-PCR
• Protein expression analysis by Western blotting, ELISA
• Immunohistochemistry and/or immunocytochemistry
• Sample preparation for MassSpec proteomics and single cell transcriptomics
• Fluorescent microscopy, image acquisition (confocal/brightfield) and analysis
• Animal work (Biological sample collection, genotyping, minor surgery and some limited animal husbandry)
• Kidney microdissection and glomerular isolations

REQUIREMENTS:
• Bachelor’s Degree in Cell and Molecular Biology or related field with more than 2 years’ experience in biomedical research bench work -or- equivalent combination of experience and educational, postdoctoral and research staff experience in human/animal models of molecular and cellular biology.
• Established record of independent execution of research projects.
• Proven experience with sterile tissue culture, animal handling, regional kidney tissue isolations, sterile minipump surgeries, and in at least 4 of the key activities listed above.
• Must have a strong understanding of the scientific principles underlying performed laboratory protocols in order to assist in technical training of junior laboratory members and undergraduate students.

Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.

DESIRED EXPERIENCE
• Master’s degree in Biological Sciences preferred.
• Able to maintain organization across multiple projects, ability to clearly communicate, trouble shoot results and previously demonstrated protocol development. Collaborative team player. Experience working in the fields of either Kidney Biology or the Biology of Aging is not required but will be considered a plus.

The Rabinovitch/Sweetwyne laboratory is committed to fostering an environment that welcomes individuals from diverse backgrounds into a collaborative, supportive and rigorous scientific working group.

Application Process:
The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process.  These assessments may include Workforce Authorization, Cover Letter and/or others.  Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.