USC's KSOM Stem Cell Biology and Regenerative Medicine is seeking a Research Lab Technician II to join Dr. Andrew McMahon’s lab.The research technician will assist postdoctoral researchers studying kidney nephron development and injury/repair models for the mammalian kidney in the McMahon Laboratory at the Eli and Edythe Broad CIRM Center.
(http://mcmahonlab.usc.edu ,http://keck.usc.edu/stemcell/ )
Education requirement:
- College background in biology, molecular biology, biotechnology or biochemistry.
- Research experience on the kidney is essential.
- Cell Culture experience with human and mouse lines is preferred.
- Bachelor’s degree is preferred.
- Experience with molecular biology techniques is preferred.
- Cloning
- PCR/qPCR
- DNA/RNA extraction
Duties will include:
- Support in maintaining mouse colonies, genotyping mice, setting up matings, embryo collection, tissue biopsy, collection of tissues for: frozen sections, in situ hybridization and immunohistochemistry and photography and computer cataloguing of data as needed.
- Cell culture maintenance, along with expansion and preservation of cell lines.
The individual will assist other researchers as assigned. Basic knowledge of molecular biology experimentation: (PCR, RNA isolation, DNA digestion, agarose gel electrophoresis) able to follow protocols for tissue fixation and processing- One or two years experience in mouse husbandry, in situ hybridization, histology, cell culture or related techniques.
Excellent organizational skills, and an ability to work independently but also as part of a team are essential requirements.
Other Job Accountabilities include:
- Conducts research experiments in accordance with laboratory and safety protocols. Collects field and/or laboratory measurements and data. Computes results for a variety of research procedures, tests and techniques.
- Maintains accurate records of procedures used to conduct experiments. Uses laboratory/computer equipment to conduct analyses of experiment data. Provides routine interpretations of analyses.
- Maintains and calibrates laboratory equipment. Performs or arranges for basic repairs. May alter equipment to meet experiment requirements. Makes recommendations for equipment acquisitions.
- Prepares chemical solutions for experimental use.
- Handles and disposes of hazardous and non-hazardous materials in accordance with safety protocols.
- Maintains and organizes laboratory supplies. Monitors inventory levels and orders or secures supplies as needed.
- Maintains research facility in orderly, operable condition. Maintains security and safety of equipment, samples, specimens and data.
- Conducts library research to assist in the planning and design of experiments.
- Learns basic laboratory safety and, if appropriate, basic radiation or blood borne pathogen safety under the direction of the USC Safety Office and more experienced laboratory staff.
- Performs other related duties as assigned or requested. The University reserves the right to add or change duties at any time
Hourly rate: Depends on experience
Minimum Education: Associate's degree, Specialized/technical training, Combined experience/education as substitute for minimum education
Minimum Experience: 2 years
Minimum Field of Expertise: Working knowledge of laboratory equipment and research protocols. Knowledge of computer analysis techniques and ability to provide routine analysis and interpretation of research data.
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