Research Associate

North Carolina State University, United States

Updated: 3 months ago
Location: Raleigh, NORTH CAROLINA
Deadline: ;

Posting Details
Posting Information


Posting Number PG193041EP
Internal Recruitment No
Working Title Research Associate
Anticipated Hiring Range Commensurate with experience
Work Schedule Monday - Friday; 8 am - 5 pm or as needed by supervisor
Job Location Raleigh, NC
Department Biomedical Engineering
About the Department
The UNC-NCSU Joint Department of Biomedical Engineering stresses the application of science and engineering, mathematical analysis, and computer techniques to biomedical problems. The research and entrepreneurship of the faculty, students, and staff aim to accumulate, generate, and disseminate biomedical engineering knowledge to improve health care for the benefit of the people of North Carolina, the nation, and the world.
Essential Job Duties
The Research Associate will be responsible for the following:
  • Mammalian cell culture: culture of established human cell lines and BSL2 primary human cells, especially endothelial cells, fibroblasts, and immune cells. Includes maintenance, expansion, and cryopreservation of cells.
  • Handling and manipulation of blood and blood products
  • Processing tissue specimens to dissociate and isolate multiple cell types
  • Preparation and maintenance of vascular microphysiological systems (MPS)
  • Fixing, immunostaining, and imaging vascular MPS
  • Confocal microscopy: acquisition of images from live and fixed MPS, time-lapses, fluorescence recovery after photobleaching (FRAP), reflectance microscopy, etc.
  • Plasmid preparation and lentivirus transfection (e.g. to produce stable fluorescent protein expression in human cells), antibiotic selection
  • Additional molecular biology techniques: RT qPCR, ELISA, Western blotting, flow cytometry, etc.
  • Data and image analysis, preparation of figures and text for publications and grant submissions
  • Purchasing and receiving
  • Environmental health and safety (EHS) compliance, documentation, inspections
  • IRB and IACUC documentation
  • Maintaining liquid nitrogen and carbon dioxide gas cylinder supplies
  • Routine equipment maintenance and supervision and training of junior lab members.
Other Responsibilities

Qualifications


Minimum Education and Experience
Relevant post-Baccalaureate degree required, preferably in a biomedical field
Other Required Qualifications
  • Aseptic mammalian cell culture technique at biosafety level 2 (potentially infectious human cells and tissue).
  • Molecular biology techniques such as ELISA, RT qPCR, Western blotting, etc.
  • Design and preparation of plasmids and viral transfection for protein overexpression.
Preferred Qualifications
  • Skills in microscopy, especially confocal, including live-cell imaging and preparation and staining for immunofluorescence imaging.
  • Experience in the isolation of cells from fresh tissue and exposure to 3D cell culture such as organoids and microphysiological systems.
  • Knowledge of vascular biology and thrombosis/hemostasis.
  • Established record of high-quality scientific publications.
  • PhD preferred
Required License(s) or Certification(s)
N/A
Valid NC Driver's License required No
Commercial Driver's License required No

Recruitment Dates and Special Instructions


Job Open Date 12/01/2023
Anticipated Close Date Open until filled.
Special Instructions to Applicants
Please include a CV and contact information for three professional references. 

Position Details


Position Number 00110506
Position Type EHRA Non-Faculty
Full Time Equivalent (FTE) (1.0 = 40 hours/week) 1.00
Appointment 12 Month Recurring
Mandatory Designation - Adverse Weather Non Mandatory - Adverse Weather
Mandatory Designation - Emergency Events Non Mandatory - Emergency Event
Department ID 147501 - Biomedical Program - ENG
AA/EEO
NC State University is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, national origin, religion, sex, gender identity, age, sexual orientation, genetic information, status as an individual with a disability, or status as a protected veteran. Individuals with disabilities requiring disability-related accommodations in the application and interview process are welcome to contact 919-515-3148 to speak with a representative at the Office of Institutional Equity and Diversity.
If you have general questions about the application process, you may contact Human Resources at (919) 515-2135 or [email protected] .
Final candidates are subject to criminal & sex offender background checks. Some vacancies also require credit or motor vehicle checks. Degree(s) must be obtained prior to start date in order to meet qualifications and receive credit.
NC State University participates in E-Verify. Federal law requires all employers to verify the identity and employment eligibility of all persons hired to work in the United States.

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