Research Assistant

Updated: 8 days ago
Location: Detroit, MICHIGAN
Deadline:

Posting Number
045224  
Position Title
Research Assistant  
Number of Vacancies
1  
School/College/Division/Institutes/Centers
06 - School of Medicine  
Primary Department
H0610-Obstetrics/Gynecology  
Essential Functions (Job Duties)
Skill in working with transgenic stem cells to upgrade and re-engineer a Patented transgenic embry-onic stem cell line (ESC). These cells report in a high throughput screen (HTS) for toxicity and metabolic stress using a stemness promoter to drive a reporter in viable stem cells. For academic purposes we have used fluorescent reporters but for commercial purposes it is important to use a luminescent reporter. FUCCI cell cycle indicator ESC are used to monitor QC of toxicant exposure in time lapse.
Skill in doing a number of validations of the new ESC HTS lineage and compare these to the validations of the current patented ESC HTS1 (Rex1 promoter-red fluorescent protein ESCs) using Biotek Synergy H1 microplate readers, Sartorius IncuCyte Zoom fluorescence live imager, FACS, immunofluorescence and fluorescence micrography, immunoblot, and Drop-seq/RNA-seq with 10X Genomics. Also, R&D for a placen- tal trophoblast stem cell (TSC) HTS2 assay. Validations will emphasize testing large sets of toxicant samples for dose-dependent (dosimetric) toxic effects on our patented ESC or TSC reporter lines. These include po- tency/stemness decrease, differentiation increase and growth rate decrease assays.
Ability to work with stem cells and manage and keep optimal their growth rates and stemness qualities is essential to performance of job duties.
Knowledge of stress dose-response dosimetry in cell culture for toxicological high throughput screens or software used to calculate IC50s, NOAEL, and LOAEL and perform other Point of Departure toxicological analyses.
Knowledge or personal experiences with as many of these specific protocols and technologies.
 
Unique Duties
 
Qualifications
In addition to the specific job duties are general areas of strength. These include ability to communicate and work with teams, effort, honesty, planning and keeping a clear lab notebook (e.g. for patent needs). Preferred are keen general skills and ability in logic, communications, and effort and specific skills in molecular biology with probable lentivirus infection strategy, embryonic stem cell culture and assays used for the validation steps.  
Preferred Qualifications
 
Testing Requirements
Not Applicable  
Test Scheduling
 
Working Conditions
 
Job Type
Fractional Time  
Job Category
Research Assistants/Associates  

Fiscal Year of Job Open Date
2020  
Job Open Date
(When job is posted in the system)

09-16-2020  
Job Close Date
(or until suitable candidate is found)

Until Suitable Candidate is Found
Is this position reposted?
No  
Reposting Reason:
 

Background Check Requirements
University policy requires certain persons who are offered employment to undergo a background check, including a criminal history check, before starting to work. If you are offered employment, the University will inform you if a background check is required.  
Instructions for submitting your application:
 
Represented Position Message
 

Salary (Minimum)
(Non-Academic Only)

 
Salary (Hire Max)
(Non-Academic Only)

 
Salary (Maximum)
(Non-Academic Only)

 
Hourly Rate
(Non-Academic Only)

 
Salary Range
(Academic Only)

 
Is Position Bump Ineligible?
(Non-Academic, Represented positions only. Select 'No' for Academic. Select N/A if Non-represented.)

**NOTE: If 'Yes' is selected, then this is a Bump Ineligible Position (BIP). The person who bids or hires into this 100% grant funded position has no bumping rights upon grant expiration or in a reduction of force.

Yes  
View or Apply

Similar Positions