Research Assistant, Department of Neuroscience – Georgetown University Medical Center

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Requirements

Research Assistant, Department of Neuroscience – Georgetown University Medical Center

Job Overview

The Research Assistant supports research related to understanding the significance of APOE glycosylation to Alzheimer’s disease pathogenesis, using induced pluripotent stem cells to understand the significance of APOE glycosylation to APOE pathogenesis.

Work Interactions and Duties

The Research Assistant, working closely with Principal Investigator and a postdoctoral fellow, receives guidance on a day-to-day basis to facilitate the goals of the project, and interacts professionally and collegially and respectfully with all lab members, including graduate and undergraduate students, treating all lab members with respect and consideration.

Duties include but are not limited to:

Cell Studies

• Maintain cell culture of induced pluripotent stem (iPS) cells
• Perform differentiation of iPS cells
• Stain iPS cells for microscopic analyses and flow cytometry
• Perform immunofluorescence imaging of iPS cells

Procedures

• Prepare samples iPS cells for analyses of a variety of biochemical analyses
• Conduct assays, including ELISA, Western blot, protein concentration assays and binding assays
• Prepare mass spectrometry samples

Analyses

• Perform quantitative PCR analyses of iPS cells
• Prepare and run genotyping analyses

Lab Support

• Order lab supplies
• Perform routine lab maintenance, such as refilling cell culture disposals, weekly safety checks
• Perform autoclaving and disposing of cell culture waste
• Participate in weekly lab meeting and project meetings

Qualifications

• Bachelor’s degree in a science or science related field
• At least 1 year of experience in a laboratory environment
• Knowledge of basic cell biology and physiology

Preferred qualifications

• Experience with tissue culture
• Any experience with biochemical or cell-based assays, and molecular biology techniques such as PCR

Work Mode: On Campus. Please note that work mode designations are regularly reviewed in order to meet the evolving needs of the University. Such review may necessitate a change to a position’s mode of work designation. Complete details about Georgetown University’s mode of work designations for staff and AAP positions can be found on the Department of Human Resources Mode of Work Designation .

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EEO Statement:

Georgetown University is an Equal Opportunity/Affirmative Action Employer  fully dedicated to achieving a diverse faculty and staff.  All qualified applicants are encouraged to apply and will receive consideration for employment without regard to race, color, religion, national origin, age, sex (including pregnancy, gender identity and expression, and sexual orientation), disability status, protected veteran status, or any other characteristic protected by law .

Benefits:

Georgetown University offers a wide variety of comprehensive and competitive benefits. Benefits packages include comprehensive health, dental and vision plans, disability and life insurance coverage, retirement savings programs, tuition assistance, voluntary insurance options (including group legal, accident, and critical illness), and much more. Whatever your need, the Office of Faculty and Staff Benefits will be standing by to support you. You can learn more about the benefits offered to eligible faculty and staff at https://benefits.georgetown.edu  or view the online interactive benefits guide  for more information.