Hydrogen/Deuterium Exchange Mass Spectrometry of Proteins and Biosimilar Drugs

RAP opportunity at National Institute of Standards and Technology     NIST

Hydrogen/Deuterium Exchange Mass Spectrometry of Proteins and Biosimilar Drugs

Location

Material Measurement Laboratory, Biomolecular Measurement Division

opportunity location
50.64.51.B6750 Gaithersburg, MD

NIST only participates in the February and August reviews.

Advisers
name email phone
Kyle Wesley Anderson [email protected] 240-314-6285
Description

This research develops and applies hydrogen/deuterium exchange mass spectrometry (HDX-MS) for determining the dynamics and physical-chemical properties of proteins, biopharmaceuticals, glycoproteins, peptides, peptide-metal complexes, receptors, and biocatalysts. Topics of particular interest include the effects of post-translational modifications (PTMs) on protein dynamic structure and protein-ligand interactions, structure-function relationships between glycan structure and glycoprotein folding, and comparability measurements among innovator drugs and biosimilar drug candidates. The HDX-MS laboratory can now conduct measurements on membrane and transmembrane proteins with fully automated apparatus. Research is conducted at the nearby NIST & UMD joint Institute for Bioscience & Biotechnology Research. The well-equipped laboratory includes a fully automated HDX Orbitrap tandem mass spectrometer with ETD capability, a cryo-EM, SAXS, neutron source, a wide variety of analytical and preparation chromatographs, and numerous optical/NMR spectroscopic instruments.

key words

Bioactivity; Biocatalyst; Biomolecule; Biosimilar; Cell signaling; Conformation; ECD; Electron capture dissociation; Electron transfer dissociation; ETD; Glycan; Glycoeptitope; Glycoprotein; Glycosylation; H/D exchange; Hydrogen-deuterium exchange; HDX-MS; HXMS; Ion fragmentation; Mass spectra; Mass spectrometry; Membrane protein; Myristoylated protein; Myristoylation; Peptide; Protein; Structure function; Tandem mass spectrometry; Transmembrane protein;

Eligibility

Citizenship:  Open to U.S. citizens

Level:  Open to Postdoctoral applicants

Stipend
Base Stipend Travel Allotment Supplementation
$82,764.00 $3,000.00