This is a 100% limited appointment for five (5) months with the possibility of extension for an additional two (2) months, depending on project needs and funding availability.
The Staff Research Associate will assist the PI and other senior researchers in research projects to determine the function of neurotransmitter switching in adult mice. Under supervision of the PI, the incumbent will perform standard experimental techniques in immunohistochemistry, in situ hybridization, confocal microscopy, stereotaxic injection of viruses, development and execution of behavioral assays including novel object recognition, novel odor recognition and contextual fear conditioning, and carry out in vivo experiments. The incumbent will manage the care and breeding of mice, providing work direction to undergraduate student laboratory assistants in caring for mice and other lab responsibilities. Additional duties include overseeing lab supply ordering and lab safety, and other duties as assigned.
Theoretical and practical knowledge of biochemistry, molecular biology, immunology and genetics, including relevant laboratory techniques, acquired through a Bachelor's Degree in Biology or related field; OR an equivalent combination of education and experience.
Proven experience with molecular biological methods and procedures including DNA/RNA isolation, purification, amplification, and detection, agarose and acrylamide gel electrophoresis, and western blotting.
Demonstrated experience in the use of lab equipment including spectrophotometer, balance, vortex, osmometer, pH meter, autoclave, drying oven, micropipette and centrifuge.
Demonstrated knowledge of embryological anatomy, ultrafine dissection, antibiotics, and sterile techniques.
Proven experience with weighing and measuring chemicals, and preparing molar and normal solutions and serial dilutions.
Thorough knowledge of mouse anatomy, care, feeding, watering and maintenance techniques. Ability to train others in these duties. Ability to perform subcutaneous injections. Knowledge of regulations affecting use, care, breeding and disposal of animals.
Strong knowledge of federal guidelines for Good Laboratory Practices for humane use of animals. AAALAC or AALAS certifications desirable. Knowledge of research animal care protocols and procedures as defined by AAALAC and AALAS, and governmental agencies, and sensitivity to the humane care of animals in research.
Demonstrated ability to train and provide work direction to others, including undergraduate students, in various standard lab experimental techniques, protocols, and procedures.
Proficient computer skills in MS Word, Excel, PowerPoint, email, etc. Ability to independently learn new programs. Skill in maintaining clear and concise records, inventory of supplies and equipment.
Ability to learn the scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.
Excellent interpersonal skills including tact, diplomacy and flexibility with the ability and experience to work in a team framework with staff, faculty and students. Ability to participate and contribute to discussions regarding procedures and goals of lab.
Excellent verbal and written English communications skills, to articulate information on one’s position, plan of action, evaluation or interpretation in a professional, logical, clear, effective, concise, and courteous manner.
Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, and use of protective items. Willingness and ability to keep safety training current and consistent with research involvement.
Ability to work safely with hazardous materials. Knowledge of proper handling, storage and disposal of chemicals, biochemicals, hazardous chemicals, and biohazardous materials including radioactive materials. Knowledge and experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Programs.
Ability to embrace and consistently uphold the UC San Diego Principles of Community for a collaborative work environment.
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