Sequencing Technician (Fixed Term) x 2

Updated: about 2 months ago
Location: Cambridge, ENGLAND
Job Type: FullTime
Deadline: 29 Apr 2021

The Stratified Medicine Core Laboratory (SMCL) is a research facility hosted by the University Department of Medical Genetics. The facility is involved in world class sequencing research partnerships with clients both within Cambridge University, Cambridge University Hospital NHS Foundation Trust and across the UK, working on matters of national and international importance in the field of life sciences and routine medical diagnostics. The facility's aim is to provide a comprehensive and expert Next Generation Sequencing (NGS) service. Our current sequencing platforms consist of, Illumina MiSeq, NextSeq500 and HiSeq4000. The facility also has a range of ancillary support hardware covering; nucleic acid extraction robotics (from blood, fresh/FFPE tissue etc), DNA shearing, liquid handling robotics, sample/library QC analysis.

We are now seeking two Sequencing Technicians to join us. The successful applicants will play a major role in delivering RNA sequencing data from major projects lead by Dr Eoin McKinney (PI, Cambridge Institute of Immunotherapy and Infectious Disease, Jeffrey Cheah Biomedical Centre) in collaboration with international consortia. The projects have established pipelines to generate and analyse genome wide transcriptomes from clinical studies in type 1 diabetes (T1D). The project aims to identify and interpret the biological and clinical significance of longitudinal changes in patterns of immune cell intrinsic gene expression during the development of T1D. Its aims are to develop predictive biomarkers facilitating early, targeted treatment and to inform our understanding of the mechanism of T1D onset and progression. In each of two major projects RNAseq data will be generated from purified immune cell populations using samples taken longitudinally from children prior to T1D and matched controls. One will generate transcriptomes using RNAseq on purified immune cell populations. The other will use single cell RNAseq combined with feature enrichment libraries including surface protein (CiteSeq) and antigen receptor (VDJ) sequencing. The post holder will also be expected to provide support to the wider laboratory team, providing a high-quality standard laboratory environment. The successful candidate will also have the opportunity to gain experience in the maintenance and operation of our NGS platforms.

Candidates should have Molecular Biology & Genomics skills, with a strong background working in Research Laboratory settings. Experience with next-generation sequencing library preparations would be desirable, however full training will be given to successful candidate(s). You should be educated to degree level, or equivalent, in a biological subject. A high level of attention to detail is required in laboratory work and previous use of SOPs and adhering to GLP is desirable. Good written and oral communication skills, and meticulous recording keeping are also essential.

Fixed-term: The funds for this post are available for 12 months from the start date in the first instance.

For formal enquiries please contact Dr Graeme Clark on email: . For informal enquiries please contact Shona Markwell, HR Coordinator on email:

This appointment requires a Research Passport application.

To apply online for this vacancy and to view further information about the role, please visit : .

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