Research Support Assistant - Structural Studies - Dr Andrew Carter - LMB 1487

Updated: about 2 months ago
Location: Cambridge, ENGLAND
Deadline: 21 Mar 2021

View Vacancy -- Research Support Assistant - Structural Studies - Dr Andrew Carter - LMB 1487
Open Date
19/02/2021, 09:00
Close Date
21/03/2021, 23:55

MRC Laboratory of Molecular Biology

The MRC Laboratory of Molecular Biology (LMB) is one of the birthplaces of modern molecular biology and has played an important role in the development of many new techniques, most notably protein crystallography, cryo-electron microscopy, DNA sequencing and monoclonal antibodies.  The LMB has a clear goal of understanding biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease.  We aim to tackle difficult, long-term problems, which often require investment over many years.
There are four scientific Divisions - Structural Studies, Protein and Nucleic Acid Chemistry, Cell Biology and Neurobiology.  However, scientific collaboration between the different Divisions is extensive.  Scientists are well supported by excellent scientific facilities and by the LMB Operations Group which maintains the core infrastructure and services of the institute.
The LMB provides an unsurpassed environment for both new and established researchers.  Scientists are drawn to the LMB from all over the world, thus creating a lively and international community for the exchange of ideas and technical innovation.
UK Research and Innovation is a new entity that brings together nine partners to create an independent organisation with a strong voice for research and innovation, more information can be found at

MRC - 5


£25,198 - £26,901 per annum

Fixed Term


Full Time

Fixed term until 31 August 2023

Overall purpose:
To assist researchers in preparing, performing, and evaluating experiments under the guidance of the group leader at the MRC Laboratory of Molecular Biology (LMB).
To work within a programme aimed at the characterization of the cytoplasmic dynein complex.  Specifically, to use baculovirus and mammalian expression systems to assist in the production of proteins that are cargos, accessory factors and regulators of dynein.  Also, to maintain cell cultures and perform CRISPR tagging.
Main duties/ Key responsibilities:
To develop strategies for the production of challenging multi protein complexes for structural and biochemical analysis.  
Responsibilities will include all steps of protein production i.e. growth and maintenance of insect and mammalian cell lines, cloning, protein expression and protein purification. They will also involve purification of complexes from native sources.
Other roles include molecular biology and CRISPR tagging of cells as well as maintaining cell cultures.
Within the objectives of the project the post holder will carry out the project with close supervision by a postdoctoral CDF.
To plan their own work and objectives on a weekly basis and will manage their experimental work within the project along with their supervisor.
To work with supervision to discuss progress and plan on a daily basis with the supervisor and members of the group.
To ensure the research is carried in accordance with good practice and in compliance with local polices and legal requirements.
To contribute to the smooth running of the research group, including the effective use of resources and taking responsibility for use of communal facilities.
To enhance their research and generic skills through a tailored development programme.
Working relationships:
You will report to the group leader and will interact and collaborate with other postdoctoral scientists, research support staff and students in the Group and across the LMB, and with external Groups as necessary.
Additional information:
The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post.  All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post and the MRC.  
It’s essential that you have or have just completed, an undergraduate or master’s degree in the biological or physical sciences or a related discipline. Interpersonal skills are required. Previous experience of laboratory research is highly desirable but training will be given.
Responsibility will be for your own work only.

Education / qualifications / training required:
BSc or higher-level qualification in a relevant field of the life sciences
Previous work experience required:
• Experience in mammalian cell culture, insect cell culture or protein purification.
• Experience working in biological research labs.
• Experience of laboratory research would be an advantage, but training will be given.

Knowledge and experience:
Independent working
Ability to plan experiments and execute carefully.
Verbal and written communication skills
Ability to communicate ideas in written and verbal form.
Personal skills / behaviours / qualities:
Enthusiastic individual with a strong desire for self-development.

The MRC is a great place to work and progress your career, be it in scientific research or the support functions.The MRC is a unique working environment where our researchers are rewarded by world class innovation and collaboration opportunities that the MRC name brings. The MRC is an excellent place to develop yourself further and a range of training & development opportunities will be available to you, including professional registration with the Science Council.
Choosing to come to work at the MRC (part of UKRI) means that you will have access to a whole host of benefits from a defined benefit pension scheme and excellent holiday entitlement to access to employee shopping/travel discounts and salary sacrifice cycle to work scheme, as well as the chance to put the MRC and UKRI on your CV in the future.
Our success is dependent upon our ability to embrace diversity and draw on the skills, understanding and experience of all our people. We welcome applications from all sections of the community irrespective of gender, race, ethnic or national origin, religion or belief, sexual orientation, disability or age. As "Disability Confident" employers, we guarantee to interview all applicants with disabilities who meet the minimum criteria for the vacancy.
UKRI supports research in areas that include animal health, agriculture and food security, and bioscience for health which includes research on animals, genetic modification and stem cell research. Whilst you may not have direct involvement in this type of research, you should consider whether this conflicts with your personal values or beliefs.
We will conduct a full and comprehensive pre-employment check as an essential part of the recruitment process on all individuals that are offered a position with UKRI. This will include a security check and an extreme organisations affiliation check.

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