Research Assistant (Dye Project)

Job Description

Fluorescent labels are essential tools in many branches of life science research. They provide high sensitivity for detection and are non-destructive to the target samples. Traditionally, fluorescent labeling is achieved by adding a chromophore to biomolecules such as proteins or nucleic acids. Conjugated oligoelectrolytes (COEs) are emerging as a new class of fluorescent molecules used to label cells and other specimens for non-invasive fluorescence imaging directly.

COEs are defined by a linear hydrophobic conjugated backbone with polar ionic pendants at both terminals. They were firstly synthesized in Prof. Bazan’s group as two-photo absorption chromophores and later found to have biological applications. COEs are membrane intercalating molecules that span the lipid bilayer. The spontaneous intercalation into membranes from aqueous media results in significant increases in fluorescence emission. By tuning the molecular framework in the conjugated core of COE, COEs characterized by different emission wavelengths can be achieved, thereby gaining access to a range of different colours for fluorescent labelling. 

This role aims to determine the labelling efficiency of various COE dyes across different biological samples, including but not limited to mammalian cells, bacteria of different membrane structures, and extracellular vesicles. The characterization and applications for these COE-labelled samples will also be investigated via different fluorescence-based techniques such as confocal microscopy, flow cytometry, and in vivo fluorescent imaging. This role will also include developing products for use as standards in fluorescent applications.

Qualifications

Requirements:

• The candidate should possess a Honours Degree
• Independent and motivated individual with good problem solving skills 
• With practical experience and/or knowledge of:
  • Biological chemistry, cell biology, or biochemistry. 
  • Characterization techniques including absorbance/fluorescence measurements, dynamic light scattering, flow cytometry or fluorescent microscopy
  • Knowledge of working with nanoparticles is highly sought after.
  • Microsoft office

Interested applicants may email your resume to chmbgc@nus.edu.sg and phwang@nus.edu.sg

More Information

Location: Kent Ridge Campus
Organization: Science
Department : Chemistry
Employee Referral Eligible: No
Job requisition ID : 20450