Research Assistant - Cellular Generation and Phenotyping

Updated: about 1 month ago
Location: Cambridge, ENGLAND

We are looking for a number of talented individuals to join the Cellular Generation and Phenotyping team (CGaP) at the Sanger Institute. You will be working as part of a diverse group using advanced gene editing technologies to deliver functional genomics projects in partnership with faculty teams.

About the role

You will join the team as a Research Assistant performing genome-wide CRISPR-Cas9 knockout screens on human cancer and induced pluripotent stem cell (iPSC) lines. As a Research Assistant within these projects, you will:

  • Perform large-scale culture of cancer and/or iPSC lines
  • Transduce these cell lines with lentivirus gRNA libraries
  • Perform quality control analysis using a range of cell-based assays such as flow cytometry and viability assays
  • Support management in reporting on project progress
  • Ensure good communication across the project team for troubleshooting and sample transfer.

About you

You will be proactive, self-organising individual to help support the expansion of CRISPR screening into new projects. We function in a collaborative environment, where you will need excellent team working, organisation and communication skills. A good knowledge of life science laboratories, particularly with an understanding of mammalian cell culture techniques, would be desirable.

You will be provided with all the appropriate training for you to fulfil your role. We encourage all staff  to take part in the learning and development opportunities available within the department and the wider Institute.

About the research

We are a core research group, responsible for providing cell biology resource and expertise to the wider institute. We do this by running multiple, diverse, high-throughput projects for faculty teams across the Sanger Institute. Our current portfolio of projects include, genome wide CRISPR screens of iPSC and cancer cells, differentiation of iPSCs toward neuronal lineages, organoid derivation, iPSC derivation and Spatial Genomics associated with the Human Cell Atlas Initiative.

The projects that these Research Assistant positions will support are part of a larger initiative to investigate gene essentiality on an individual patient level. The outcomes of these projects will contribute to the understanding of cancer biology and patient-to-patient variability. The overall aims are to support the faculty groups to identify novel cancer drug targets.

Further information about CGaP, and the projects we work on, can be found on our website:

Please include a CV and covering letter with your application describing how you meet the essential criteria for the role.

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