<!--anchor--> <!--anchor--> Research Associate - INTERNAL ONLY <!--anchor-->

THIS VACANCY IS OPEN TO INTERNAL APPLICANTS ONLY

The group of Dr Marc-David Ruepp is seeking a Research Associate to carry out a project to investigate the molecular determinants of FUS toxicity in FUS-linked ALS.  

We are looking for a motivated and dedicated candidate with good knowledge in RNA biology, specifically pre-mRNA splicing, RNA-IP, stem cell and general mammalian cell culture as well as genome editing. 

The group is based within the UK Dementia Research Institute (DRI) Centre that is embedded within the Department of Basic and Clinical Neuroscience at King’s College London www.kcl.ac.uk/ioppn/depts/bcn/dri/lab-groups . The DRI is a national consortium focused on cutting-edge dementia research funded by the Medical Research Council, Alzheimer’s Society and Alzheimer’s Research UK (www.ukdri.co.uk).  

The key objective of this post is the generation of cellular and in vivo models of FUS-linked ALS. The post holder will be expected to take a lead role in the generation and characterization of these models and deliver cutting edge research output.  

This post will be offered on  a fixed-term contract for 2 years

This is a full-time  post - 100% full time equivalent

• Generation of FUS variants by site directed mutagenesis / molecular cloning
• Expression of recombinant proteins
• Testing of variants in RNA-binding and phase separation assays
• Perform functional validations of generated models
• Find innovative solutions to potential problems
• Liaise with postdocs and PhD students of the group to ensure efficiency and reproducibility
• Work in a collaborative manner, sharing knowledge and expertise within the team, department and with collaborators
• Undertake any other reasonable duties that may be requested by the PI.

The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.

Essential criteria

• PhD awarded in relevant area of Neuroscience or Molecular Biology/Biochemistry 
• Knowledge in RNA Biology 
• Knowledge in Human cell culture 
• Knowledge in Molecular Biology 
• Knowledge in Pre-mRNA splicing 
• Knowledge in molecular mechanisms of neurodegeneration 
• RNA-IP 
• PCR/RT-qPCR 
• Western Blotting 
• Mammalian cell culture 
• Stem cell culture 
• Genome editing using CRISPR/Cas9 
• Recombinant protein expression 
• MST 
• Immunofluorescence 
• Biochemistry/Molecular Biology 
• Dynamic and highly motivated 
• Ability to work proactively 
• Able to work independently and in a team 

Desirable criteria