Advanced Research Assistant (Mammalian Cell Culture) - Gene Editing

Updated: about 2 months ago
Location: Cambridge, ENGLAND

We have a new opportunity for a Advanced Research Assistant to join the Gene Editing team.

Do you have a strong background in mammalian cell culture? If yes, this could be the role for you. 

About the role:

  • You will be responsible for the design, execution and validation of gene editing experiments, this will range from gene knockouts and single base substitutions to larger knock-in projects.
  • You will be expected to independently lead these projects, working towards an agreed timeline, ensuring accurate record keeping and communicating progress to key stakeholders.
  • You will work closely with other members of the team, and contribute to troubleshooting discussions and the general success of other projects.
  • You will work on ambitious cross-functional projects with the wider CellOps division, such as saturation genome editing.
  • You will be actively encouraged to seek out developmental opportunities and work with senior staff to test and implement novel approaches.

About you:

  • You will have a strong background in mammalian cell culture, with a BSc degree in the relevant subject as well as practical experience.
  • You will be a highly motivated scientist, capable of working independently, troubleshooting your work and disseminating results to a wide range of individuals.
  • You will thrive in collaborative environments and be eager to both support others and take advantage of the ample experience within the group.
  • You will also be highly motivated to stay up-to-date with the latest technologies and implement new methods to ensure that we continue to support cutting-edge research.

About us:

We facilitate the Wellcome Sanger Institute’s world leading genomic research by providing the expertise and resources required to deliver advanced genome engineering projects, that enable faculty researchers to focus on their scientific objectives.

The two main services that we provide are: cellular engineering, using bespoke gene editing approaches to generate novel cell lines, and custom vector construction, providing high quality gene delivery and gene editing tools. 

For more information about our team visit: www.sanger.ac.uk/group/gene-editing/ 


Essential Skills
  • BSc in a Biology related subject
  • Extensive practical experience in mammalian cell culture, ideally pluripotent stem cells
  • Familiar with cellular phenotyping assays such as FACs, immunofluorescence and qPCR
  • Strong theoretical understanding of CRISPR/Cas technology and cell line generation
  • Enjoy working collaboratively, supporting members of the team and sharing results in an open and timely manner
  • Diligently maintain accurate records of experiment
  • Strong communication skills, confident in disseminating information to various audiences
  • Work independently, once given adequate training, capable of managing workloads and working to deadlines
  • Proactive in troubleshooting, developing protocols and implementing new strategies

Ideal Skills
  • Experience generating gene edited cell lines or model organisms (D)
  • Experience in gene delivery methods, such as electroporation, viral transduction and lipid-based transfection, and associated genotyping (D)
  • Confident in the use and interpretation of standard genome browsers, e.g. Ensembl, and molecular biology software, e.g. SnapGene (D)

Other information

Due to Covid 19, the Wellcome Genome Campus closed for all but essential functions and facilities. As we begin restarting our science, we have implemented measures to support our staff on site including one way systems, enhanced cleaning and increased facilities for handwashing. You can learn more about our campus and our return to research here . This role will require working on site and full details of working on site can be discussed during the interview stage.

Interviews will be taking place virtually.

Closing date: 25th April, 2021


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