Staff scientist in volume imaging

The Uhlén/Mulder research group drives several efforts within the human protein atlas with a special focus on brain and comparisons between mammalian species. The group uses a large variety of methods to identify protein expression and distribution in tissues (mainly brain) under normal ‘healty’ conditions or in disease. The used methods include next generation sequencing techniques (bulk, single cells and spatial), 2D immunofluorescence and 3D volume imaging. The group consists of senior researchers (staff scientists), research assistants (PhD candidates) and BSc, MSc and PhD students. In additions there are many close collaborations with other research groups within the Human Protein atlas consortium.

The human protein atlas project aims to identify protein expression and protein distribution in all human tissues. For this an antibody based approach is used, staining sections of various tissues and disease conditions. Some tissues with a complex cellular composition or complex and heterogeneous disease processes 2D methods are not sufficient. Our biological laboratory performs volume imaging (3-dimensional) analysis of healthy and disease-affected human brains and other peripheral tissues using the IDISCO technique combined with light sheet microscopy. We are looking for a candidate who can independently continue this line of research and complete ongoing projects. The newly employed staff scientist will perform volume imaging experiments and light sheet (3D) microscopy and will analyse collected data. Furthermore, the research should also interact with collaborators within the human protein atlas project, oversee tissue preparation, oversee and perform wet lab experiments, perform primary interpretation of results, and be involved in scientific discussions and publication of data. The job involves minor teaching activity and supervision of master students.

We are looking for a scientist with PhD degree, who has substantial experience with the anatomy of the human brain and peripheral (particularly vegetative sympathetic) nerves, with volume imaging- and with histological techniques in general. The ideal candidate has scientific knowledge and earlier experience in the field of neurodegeneration, central/peripheral monoaminergic innervations, and brain mapping. The applicant should have experience using IDISCO methods on human tissue samples and should be able to operation a light sheet microscope. Experience in 3D image analysis, using Imaris (including XT and filament tracer modules) is desired but not required. Advanced English, good skills with problem solving and scientific writing, and affinity to teaching are essential. The candidate should also have a track record showing the ability to write publications of high quality and impact. Only candidates that authored at least ten papers on neuroscience, neurodegeneration, peripheral mono-aminergic innervation and or volume imaging of which at least two as first, last or communicating author in journals with impact factor >10 are considered. Publication of volume imaging data based studies  in high impact journals is considered an advantage.

Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote better health for all. At Karolinska Institutet, we conduct innovative medical research and provide the largest range of biomedical education in Sweden. Karolinska Institutet is a state university, which entitles employees to several benefits such as extended holiday and a generous occupational pension. Employees also have free access to our modern gym and receive reimbursements for medical care.

Location: Solna

Application

The application is to be submitted through the Varbi recruitment system. In this recruitment, you will apply with your CV without a personal letter. Instead, you will answer some questions about why you are applying for the job in the application form.