Do you want to contribute to top quality medical research?
The Eukaryotic Single Cell Genomics facility (ESCG), is looking for a highly qualified Research Specialist.
Description of the subject area
ESCG is a core facility at Karolinska Institutet and a national facility at Science for Life Laboratory (SciLifeLab). Based at SciLifeLab in Stockholm, the focus of ESCG is on high throughput streamlined transcriptome and genome analysis of large numbers of individual eukaryotic cells.
ESCG constructs RNA and DNA sequence libraries from single-cell suspensions using state-of-the-art technologies. You will handle delicate primary cell samples, fragile RNA and sensitive instruments on a daily basis.
Your responsibilities will include all aspects of running single-cell methods, RNA-isolation and sequencing library construction, microscopy, image analysis, quality control, trouble-shooting. Methods include Smart-seq3 protocol, Chromium system from 10x Genomics and custom protocols. Additionally, you will operate liquid handling robots, qPCR/PCR, and Bioanalyzer instruments. Your work may also include to implement new single-cell methods at the facility.
Other tasks may include project coordination, user consultations, cost calculations, invoice preparations, development of laboratory protocols (SOPs), procurement, ordering, shipments, inventory, risk assessments.
You are service-minded and enjoy learning new methods. You are thorough, flexible, diligent in your laboratory work and proficient in work documentation. The position requires being able to work independently as well as in collaboration with others. You are able to work with several projects in parallel. You will work in an international team with frequent interactions with customers, therefore clear and informative communication skills, general computing proficiency, and a good knowledge of English are essential. Strong interpersonal skills will be highly valued.
To be eligible for employment as a Research Specialist, in addition to holding a Degree of Doctor, PhD, or having equivalent scientific expertise, the applicant must have demonstrated research expertise as well as have documented research experience after defending their doctoral dissertation.
- Ph.D. in molecular biology, biomedical science, biotechnology or similar discipline
- Previous work with RNA and primary cells
- Good knowledge in molecular biology techniques
- Communication in English
- Experience with laboratory work following Good Laboratory Practice (GLP), including biosafety rules
- Previous work with single-cell techniques
- Method development in the molecular biology field
- Experience with high-throughput sample preparation of RNA and/or DNA
- Experience with laboratory automation
- Experience with data handling, programming and bioinformatics
- Experience with a LIMS system
- Experience with NGS library prep
After an overall assessment of the expertise and merits of the applicants in relation to the subject area, Karolinska Institutet will determine which of them has the best potential to contribute to a positive development of the activities at KI.
What do we offer?
A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Here, we conduct successful medical research and hold the largest range of medical education in Sweden. At KI, you get to meet researchers working with a wide range of specialisms and methods, giving you ample opportunity to exchange knowledge and experience with the various scientific fields within medicine and health. It is the crossover collaborations, which have pushed KI to where it is today, at the forefront of global research. Several of the people you meet in healthcare are educated at KI. A close relationship with the health care providers is important for creating groundbreaking top quality education and research. Karolinska Institutet is also a state university, which entitles you to several benefits through our collective agreement.
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We prefer that Your application is written in English, but You can also apply in Swedish. An application must contain the following documents: Resumé, qualifications and description of planned research, presented in accordance with Karolinska Institutet's qualifications portfolio.
Welcome to apply.
The application must be submitted through the Varbi recruitment system.
Want to make a difference? Join us and contribute to better health for all
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