Research assistant in Molecular biology

The Department of Laboratory of Medicine, located in Huddinge Campus, is one of Karolinska Institutet’s largest departments with seven research divisions including Biomolecular and Cellular Medicine (BCM), where the project will be carried out.
350 people are employed at the department, half of which are PhD-students. The Centre provides the facilities needed for cell- and molecular biology research and the possibility to perform research in vivo.

The Zain-Smith lab works on two major projects, one related to neurodegenerative disorders and oligonucleotide therapy and the other related to B lymphocyte development and the BTK tyrosine kinase. Mutations in BTK cause X-linked agammaglobulinemia and inhibitors of BTK have become important medicines for the treatment of leukemia and lymphoma. The research aims at understanding the role of BTK in cells and especially how the inhibitors function, including the analysis of resistance mutations.

We are seeking a highly motivated person to join the group of Zain-Smith to work on the analysis of BTK and of BTK inhibitors.

Most of the research will be conducted utilizing cultured cell lines and animal models. The work involves transfection of cells with various BTK mutated constructs and the analysis of how they perform. Cells are activated and the intracellular signaling is monitored using western blotting or FACS analysis of BTK substrates. The main posttranslational modifications are phosphorylation, mainly tyrosine phosphorylation.

The person is eligible for a position as Forskningsassistent if he or she has obtained a PhD.

The applicant should have a solid background in work with cells and analysis of proteins (Immunoblotting, ELISA, and IP) and RNA (qPCR). Extensive experience in cell culture work, protein biochemistry and small-molecule interactions is essential, and skills in different microscopy techniques is advantageous. The project will include work on cells from animals.

In addition, excellent proficiency in both spoken and written English is essential. We are looking for an energetic and passionate person who fits in, and actively contributes to our team.

A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement, such as: extended holiday and generous occupational pension. Employees also have access to our modern gym for free and receive reimbursements for medical care. You will also get to practice freely in our modern wellness facilities, where trained staff are on site.

Location: Flemingsberg

Choose to work at KI – Ten reasons why

An employment application must contain the following documents in English or Swedish:

-A complete resumé, including date of the thesis defence, title of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work
-A complete list of publications
-A summary of current work (no more than one page)

Welcome to apply at the latest 2022-03-30.

Permanent position is initiated by a six months trial period.