Lab Technician

Updated: 22 days ago

Do you want to contribute to top quality medical research?

Research group Holmin works with the development of new methods for endovascular selective administration and sampling as well as with molecular imaging. In addition, clinical and translational studies in stroke are conducted. We have a number of collaborations nationally and internationally with different groups and work interdisciplinary.


Your mission
  • Maintenance and expansions of various cell lines in culture, including but not limited to human embryonic stem cells (hESCs), adult stem cells such as Mesenchymal Stem/Stromal Cells (MSCs), Hematopoietic Stem Cells (HSCs). Occasionally primary mouse cell lines.
  • Performing Differentiation protocols to generate cardiac subtypes from hESCs
  • Assist lab members with experiments related to cell culture work including but not limited to: generation of transgenic cell lines, immunofluorescence, flow cytometry, fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS). Depending on project work load, you may also be asked to assist with PCR, ELISA, Western blot, molecular cloning, tissue processing and staining.
  • Lab maintenance such as: cleaning of cell culture facilities including hoods, water baths, work surface, shelving, regular mycoplasma testing of cultured cell lines and routine sterilization of CO2 incubators.
  • Assist lab manager as needed with inventory, orders and distribution of general supplies
  • Writing technical reports in the form of maintaining an electronic notebook

Please note that this position involves cell culture work, which requires flexible working hours. You will be expected to perform cell maintenance work on one weekend day of your choice and will receive the corresponding amount of time off on a week day of your choice.


Your profile

We expect you to be a team player and service minded. In addition, we are looking for a positive, flexible person with good communication skills and abilities to collaborate and work in a highly dynamic international research team. You need to be able to work both in a team as well as independently. Your work is thorough and diligent, and you are good at documenting your work and working in a clean and precise way. Interpersonal skills will be highly valued.

Requirements

  • Bachelors or a Master’s degree in a relevant scientific area.
  • Extensive experience in cell culture work, with excellent sterile handling techniques. Basic skills include cell passaging, freeze/thawing, general maintenance of cell lines, and flow cytometry.
  • Excellent communication skills in English (writing and speaking), and ideally also in Swedish.

Meritorious

  • Other related skills such as cell transfection/electroporation/nucleofection and basic molecular biology skills will be highly valued.
  • Experience with successfully and independently running even a small project from beginning to end including basic interpretation of your data with reportable results would be greatly valued.
  • Prior industry or biotechnology work experience and/or experience in GMP practices are highly desirable.
  • Experience in culturing of either human embryonic stem cells, or other stem cells such as mesenchymal stem cells, will be highly valued.

What do we offer?

Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote better health for all. At Karolinska Institutet, we conduct innovative medical research and provide the largest range of biomedical education in Sweden. Karolinska Institutet is a state university, which entitles employees to several benefits such as extended holiday and a generous occupational pension. Employees also have free access to our modern gym and receive reimbursements for medical care.

Location: Solna

Read more about the research group you will be part of  

Read more about the Department of Clinical Neuroscience  


Application

Welcome to apply at the latest April 21, 2021.

The application is to be submitted through the Varbi recruitment system.  


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