Development of CRISPR\Cas9 genomic editing tools targeting genes coding for gustatory receptors.
Transgenesis and phenotypic screening of transformants.
Preparation of RNA libraries for single-cell transcriptomic analyses.
Performing statistical analysis.
Dissemination of results in the form of articles and presentation at conferences.
The postdoc will work within the framework of the ANR BITTERTOX project which aims to understand how the evolution of the perception of the bitter taste of toxic substances can influence the evolution of behavioral and detoxification processes. we have identified a few candidate genes through a meticulous screening of genetic and phenotypic variations both within and between species of the Drosophila melanogaster subgroup. The mission of the postdoc is to validate using advanced functional genomics tools (eg: CRISPR\Cas9, single-cell transcriptomics, etc.) the possible pleiotropic effects of identified genes on perception, behavior and/or performance.
The position is funded by the ANR BITTERTOX project and will be carried out under the direction of Amir Yassin and in collaboration with Frédéric Marion-Poll, both within the Evolution, Genomes, Comportement et Ecologie (EGCE) laboratory in Gif-sur-Yvette, France.
The EGCE laboratory has a long tradition of working on the evolutionary genetics and ethology of Drosophila species. This tradition has recently been strengthened by the recruitment of young researchers whose interests range from comparative and population genomics to the developmental and neurological genetics of multiple insect models. EGCE recently joined two other research units to form the Institut Diversité, Ecologie et Evolution du Vivant (IDEEV) in Gif-sur-Yvette. The postdoc will benefit from an intellectually rich environment with state-of-the-art facilities in genome sequencing, gene editing (e.g. CRISPR\Cas9), insect breeding and experimentation, microscopy and electrophysiology, and bioinformatics analyses.
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