Artificial cells are biomimetic models used to better understand biophysical and biochemical processes. The bottom-up assembly of a minimal synthetic cell allows to reproduce the functions of a cell from molecular components. The bioengineering of new in vitro models is aimed at the development of new assays for the screening of therapeutic targets. The automation of high-throughput artificial cell manufacturing therefore aims to rapidly identify pharmacological inhibitors of biomolecules involved in different cellular functions associated with different pathologies (such as cancer).
In this thesis project, artificial cells will be reconstituted in vitro with giant lipid vesicles, mimicking the cell membrane, and with different purified cell components. The cell components will be encapsulated in the vesicles by the microinjection method. The self-assembly of cellular components, such as actin filaments (involved in cell migration), focal adhesion points (involved in cell adhesion) and microtubules (involved in cell trafficking) will be monitored using FRET (Förster Resonance Energy Transfer) nanosensors. The development of these nanosensors will make it possible to visualize the assembly of different molecules and to identify the interactions between them. The use of these nanoprobes, composed of quantum dots, will allow the detection of up to 5 biomolecules simultaneously by multiplexed FRET-FLIM (Fluorescence Lifetime Imaging Microscopy) imaging.
This interdisciplinary project will provide new perspectives in the fields of nanotechnology through the use of novel FRET nanosensors, biochemistry by establishing molecular assembly. The in vitro synthetic cell system established can then be adapted to other cellular processes.
Funding category: Sans financement dédié
The candidate have to apply for a funding from the ministry of research
PHD title: PhD in Electronics and optoelectronics, nano- and microtechnologies
PHD Country: France
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