The position requires experience in the following areas:
-Biological samples handling,conservation and processing as well as recovery methods.
-Isolation and culture of primary cells and cell lines.
-Familiarity with methodologies for the isolation of genomic DNA (nuclear and mitochondrial) and proteins from joint tissues.
-Molecular Biology Techniques (qRT-PCR)
-Immunological techniques for the quantification of proteins (ELISA, Western Blot ...)
-Immunocytochemistry and immunofluorescence techniques.
-Techniques for the study of cellular metabolism.
-Management of bioinformatics tools for the analysis of the results of each of the previously exposed techniques (qBase +, ImageJ, BD Cell-Quest Pro, etc.)
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