30 Dec 2023
Job Information
- Organisation/Company
NATIONAL UNIVERSITY OF SINGAPORE- Research Field
Chemistry- Researcher Profile
Recognised Researcher (R2)
First Stage Researcher (R1)- Country
Singapore- Application Deadline
28 Jan 2024 - 00:00 (UTC)- Type of Contract
Other- Job Status
Full-time- Is the job funded through the EU Research Framework Programme?
Not funded by an EU programme- Is the Job related to staff position within a Research Infrastructure?
No
Offer Description
Job Description
Non-viral transfection agents are essential components in RNA-based therapeutics and diagnostics. Consider for example that the recently developed vaccines for preventing COVID-19 caused by SARS-CoV-2 rely on lipid nanoparticle (LNP) formulations that stabilize mRNA and promote its desired immune response. While LNPs have been largely successful in the control of the COVID-19 pandemic, there are still many issues to improve on including off-target effects, stability, and toxicity. Conjugated oligoelectrolytes (COE) are defined by a linear hydrophobic conjugated backbone with polar ionic pendants at both terminals. The modular design of COEs allows for rapid tuning of molecular units to access a large continuum of properties. They were first synthesized in Prof. Bazan’s group as two-photo absorption chromophores and later extended to several biological applications such as antimicrobial agents and extracellular vesicle dyes.
In this project, we are interested in developing novel LNP formulations incorporating COEs with a view toward theranostic applications in vaccine development. Routine assays will be conducted on the LNP, including particle characterizations (DLS, cryo-TEM, and encapsulation efficiency), transfection, cellular uptake, and confocal imaging of intracellular fates. The performance of the novel LNP formulations will be routinely compared to commercial agents. The photophysical characterizations for these COE-LNP formulations will also be investigated via different spectroscopy-based techniques such as UV-Vis absorbance and fluorescence.
This project will be closely mentored by a postdoctoral research fellow and principal investigator, Prof Bazan.
Qualifications
- Bachelor’s degree in chemistry or related discipline.
- Independent and motivated individual with good problem-solving skills.
- With practical experience and/or knowledge of
- Biological chemistry, cell biology, or biochemistry.
- Biomaterials/materials science.
- Characterization techniques including absorbance/fluorescence measurements, flow cytometry, or confocal microscopy.
- Mammalian cell culture techniques.
- Knowledge of lipid nanoparticles and drug/gene delivery is highly sought after.
- MS office.
More Information
Location: Kent Ridge Campus
Organization: Science
Department : Chemistry
Job requisition ID : 22495
Requirements
Additional Information
Where to apply
- Website
https://www.timeshighereducation.com/unijobs/listing/361843/research-assistant-…
STATUS: EXPIRED
Similar Positions
-
Post Doctoral Fellow In Drug Delivery, Nanomedicine & Advanced Therapeutics , ;, Hong Kong, 27 days ago
We now invite applicants to join the Science, Technology, Engineering, and Mathematics Lab of Drug Delivery, Nanomedicine & Advanced Therapeutics at the Department of Pharmacology and Pharmacy, LK...
-
Postdoctoral Position In Bacterial Cell Wall Biology And Genetics , Umeå University, Sweden, 3 days ago
Umeå University is one of Sweden’s largest higher education institutions with over 37,000 students and about 4,700 employees. The University offers a diversity of high-quality education and world-...
-
Postdoc Position In Natural Killer Cell Biology And Cell Therapy , University of Oslo, Norway, about 14 hours ago
8th May 2024 Languages English English English Postdoc position in natural killer cell biology and cell therapy Apply for this job See advertisement The Precision Immunotherapy Alliance (PRIMA) PR...
-
Postdoc Position In Natural Killer Cell Biology And Cell Therapy, University of Oslo, Norway, about 8 hours ago
powered by: Cookie Information Nettsiden bruker cookies Vi ønsker at du skal være trygg når du bruker dette nettstedet. Vi benytter cookies for å sikre at du får en best mulig brukeropplevelse og ...
-
Swayne Lab – Post Doctoral Researcher In Cellular And Molecular Neuroscience, Canadian Association for Neuroscience, Canada, about 16 hours ago
Project: The post-doctoral researcher will investigate cross-cellular mechanisms regulating dendritic spine stability. Experiments will involve transgenic models both in situ and in vitro, a varie...
-
Postdoctoral Researcher Nanostructured Materials For Photovoltaic Energy Group, ICN2, Spain, about 13 hours ago
The mission of the Catalan Institute of Nanoscience and Nanotechnology (ICN2) is to achieve the highest level of scientific and technological excellence in Nanoscience and Nanotechnology. Its rese...