Assistant professor

19 Jan 2024
Job Information

Organisation/Company

Warsaw University of Technology

Department

Chair of Drug and Cosmetics Biotechnology at the Faculty of Chemistry

Research Field

Biological sciences

Researcher Profile

Recognised Researcher (R2)

Country

Poland

Application Deadline

18 Feb 2024 - 16:00 (Europe/Warsaw)

Type of Contract

Temporary

Job Status

Full-time

Hours Per Week

40

Offer Starting Date

1 Apr 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

The candidate should have a PhD degree in biology, biochemistry or related field. The candidate is expected to have experience in at least two of the research directions listed below:

- microbial genome editing using CRISPR/Cas technique

- molecular biology involving  microorganisms (bacteria, yeast cells) working with DNA/RNA

- Experience in using PCR/RT-PCR techniques

- enzymatic assays

- analysis of PHB and PLA polymers

- extraction of polymers from microorganisms and fungi and chromatographic analysis of polymers analysis

His/her scientific achievements should be documented by the authorship of papers  related to the profile of the announced research position. Capability of leading independent research activity is a necessary.

 

The project will be focused on obtaining genetically modified strains of S.cerevisiae  and their characterization. Genetic manipulations will require to design and prepare constructs for   CRISPR/Cas genome editing of S.cerevisiae. Genetic manipulations are aimed at obtaining a strain capable of simultaneouse biosynthesis of mixed  PHB/PLA (poly((R-3 hydroxybutyrate/polylactide) polymers. As part of the research conducted so far, the project leader's team has developed a strain with a favorable metabolic profile, which will serve as the starting point for genetic manipulation and for the production of the polymers. The effects of genome editing will be verified at the level of transcription using real-time PCR, at the level of metabolism by determining concentration of intracellular metabolites and in the culture media, as well as at the level of activity of selected enzymes, in the synthetic pathways as well as their cellular levels using the Western Blot immunodetection method. In the case of the most promising strains, quantitative tests of the synthesis of polylactide and hydroxybutyrate will be carried out, as well as optimization of polymer production efficiency using a laboratory bioreactor. Extraction and characterization of polymers in terms of their composition will be carried out using chromatographic methods and MALDI-TOF mass spectrometry/ NMR. Optimization of the polymer extraction process from S.cerevisiae will be a key step in the project, that will be followed by evaluation of the strategy in the design of a synthetic hybrid microorganism as an efficient polymers producer. The project will be caried out in a few interactions to obtain the optimal solution. The postdoctoral researcher will be working in a friendly environment at the Chair of Drug and Cosmetics Biotechnology at The Faculty of Chemistry WUT.

Requirements

Research Field

Biological sciences

Education Level

PhD or equivalent

Additional Information
Additional comments

Candidates are required::

1) to have completed higher education with a master’s degree or equivalent;

2) possession of a PhD in biochemical or biological sciences, or related field;

3) very good knowledge of English

4) high motivation for scientific work, independence, creativity, good organization skills;

5) documented scientific achievements in the form of publications in peer reviewed journals;

6) experience in at least two of the research directions listed below:

- microbial genome editing using CRISPR/Cas technique

- molecular biology involving  microorganisms (bacteria, yeast cells) working with DNA/RNA

- Experience in using PCR/RT-PCR techniques

- enzymatic assays

- analysis of PHB and PLA polymers

- extraction of polymers from microorganisms and fungi and chromatographic analysis of polymers analysis

 

For more information contact: [email protected]

Work Location(s)

Number of offers available

1

Company/Institute

Warsaw University of Technology the Faculty of Chemistry

Country

Poland

City

Warsaw

Postal Code

00-664

Street

Noakowskiego 3

Geofield

Where to apply

E-mail

[email protected]

Contact

City

Warsaw

Website

http://www.pw.edu.pl

Street

Noakowskiego 3

Postal Code

00-664

E-Mail

[email protected]

STATUS: EXPIRED