We are looking for a research technician Malaria Vaccinology to join our antigen discovery team!
Malaria, caused by an infection with Plasmodium falciparum parasites, continues to be one of the most devastating diseases causing almost 500,000 deaths annually, mainly young children in sub-Saharan Africa. The parasite has a complex life cycle, alternating between human and mosquito hosts. Currently, there is only one approved malaria vaccine available. However, this vaccine only provides partial protection illustrating the need to identify new vaccine candidates.
The team aims to isolate and characterize human monoclonal antibodies that block parasite invasion of liver cells and use these mAbs to identify new vaccine candidates. In this project, B-cells from immunized volunteers will be sorted, stimulated and supernatants will be screened for reactivity with parasites. After sequencing of positive B-cells, monoclonal antibodies will be produced and functionally characterized in in vitro and in vivo assays.
You will provide practical support throughout the project and will be involved in cloning antibodies, antibody production and characterisation, cell culturing, flow cytometry, DNA extraction and parasite quantification by qPCR.
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