Postdoc Cellular Neuroscience: Molecular mechanisms of fast signaling in human neurons
Postdoc Cellular Neuroscience: Molecular mechanisms of fast signaling in human neurons
Are you a motivated researcher in Cellular Neuroscience? Would you like to work on human iPSC-derived neurons and CRISPRa/CRISPRi-mediated molecular interventions? Please apply at Vrije Universiteit Amsterdam (VU).
The Integrative Neurophysiology (INF) division at the Center for Neurogenomics and Cognitive Research (CNCR) at VU offers a 3-year postdoctoral research project. The focus of the project is on elucidating molecular mechanisms of fast neuronal signaling. A critical requirement of fast neuronal computation and cognitive function is the ability of neurons to generate and maintain fast and stable output - action potentials. Because of difficult access to living human neurons, their function in supporting cognition remains largely unexplored. Our lab has extensive experience in studying the function of human neurons from neurosurgery patients. We recently showed that fast electrical signaling in human neurons directly links to cognitive ability in the same individuals (refs 1,2 below). In the current project, we aim to elucidate molecular mechanisms that underlie fast signaling by manipulating expression of specific genes in human neurons, in organotypic slice cultures as well as human iPSC-derived neurons using CRISPRa/CRISPRi (3,4). We will target genes that have been associated with cognitive ability in human subjects (5). Thereby, we aim to reveal what molecular mechanisms enable human neurons to achieve fast cellular computation ultimately supporting human cognition. As a PhD candidate, your research will aim to uncover the cellular and molecular mechanisms underlying fast electrical signaling in human cortical neurons.
The project is funded by an ERC advanced grant awarded to Prof.dr. Huib Mansvelder: https://inf.cncr.nl/research_teams/
Your duties During the research you use the following approach:
- you culture human iPSC-derived neurons and organotypic slices of human brain tissue
- you use CRISPRa/CRISPRi tools to manipulate target genes in human neurons, both iPSC-derived as well as in organotypic slice cultures of human brain tissue
- you study electrophysiological recordings and analysis from human (iPSC-derived) neurons in cultures
- you perform biochemical, transcriptomic and immunohistological analyses of expression in human neurons
- max. 38 hours per week
- €2960—€4670 per month
- Amsterdam View on Google Maps
Vrije Universiteit Amsterdam (VU)
- you have a PhD degree in molecular/cellular neuroscience, awarded between 2020 and 2023
- you are the first author on publications that are relevant to the topic of this vacancy
- you have prior experience with culturing human iPSC-derived neurons/organoids
- you have prior experience using CRISPRa/CRISPRi or other RNAi tools
- you have prior experience in cellular electrophysiology (patch clamp)
- you have excellent communication and writing skills in English
- programming and data analysis skills (Matlab, Python) are an asset
Conditions of employment
Fixed-term contract: 1 year.A challenging position in a socially involved organization. You will be part of a collaborative, enthusiastic team consisting of multiple PhD-students and experienced post-docs in which we collectively work on important research questions.
On full-time basis the remuneration amounts to a minimum gross monthly salary of €2,960 (scale 10) and a maximum €4,670 (scale 10), depending on your education and experience. The job profile: is based on the university job ranking system and is vacant for 1 FTE.
The appointment will initially be for 1 year. After a satisfactory evaluation of the initial appointment, the contract will be extended for a total duration of max 3 years.
Additionally, the VU Amsterdam offers excellent fringe benefits and various schemes and regulations to promote a good work/life balance, such as:
- 8% holiday allowance and 8.3% end-of-year bonus
- contribution to commuting expenses
- optional model for designing a personalized benefits package
Vrije Universiteit Amsterdam
The ambition of Vrije Universiteit Amsterdam is clear: to contribute to a better world through outstanding education and ground-breaking research. We strive to be a university where personal development and commitment to society play a leading role. A university where people from different disciplines and backgrounds collaborate to achieve innovations and to generate new knowledge. Our teaching and research encompass the entire spectrum of academic endeavor - from the humanities, the social sciences and the natural sciences through to the life sciences and the medical sciences.
Vrije Universiteit Amsterdam is home to more than 30,000 students. We employ over 5,500 individuals. The VU campus is easily accessible and located in the heart of Amsterdam's Zuidas district, a truly inspiring environment for teaching and research.
We are an inclusive university community. Diversity is one of our most important values. We believe that engaging in international activities and welcoming students and staff from a wide variety of backgrounds enhances the quality of our education and research. We are always looking for people who can enrich our world with their own unique perspectives and experiences.
The Faculty of Science
Working at the Faculty of Science means working together with students, PhD candidates and scientists who are focused on their discipline, yet also have a broad view of the world. We are proud of the collegial working environment within the faculty, which is characterised by an ambitious and pragmatic attitude with commitment to the bigger picture. At the Faculty of Science, scientists and students work on fundamental and complex social issues for a sustainable and healthy future. From forest fires to big data, from obesity to malnutrition, from genetics to pharmaceuticals and from molecules to the moon: our teaching and research cover the full breadth of the natural sciences. Scientific teaching and research is highly experimental, technical, computational and interdisciplinary in nature. This is why we collaborate extensively with leading research institutes and industry. The faculty has over 11,000 students studying across 40 educational programmes and employs over 1,600 staff spread over 10 scientific departments, making us the largest science faculty in the Netherlands.
Center for Neurogenomics and Cognitive Research (CNCR)
CNCR includes the Department of Neurosciences at the Faculty of Sciences. The CNCR is a vibrant scientific environment that generates integrated research programs from genes to behavior, each combining mouse and human studies (https://cncr.nl/ ). In our center, 150 people work closely together in defining how in essence simple molecular and cellular processes shape the emergent complexity of the brain.
We have the specific ambition to unravel the mechanisms by which brain cells and circuitry act both in health and disease. We aim to translate mechanistic knowledge into understanding of (dys)function of the human brain. Our neuroscience research area covers analysis over many spatial levels, from genes to the intact organism, and over a large temporal scale, from microsecond molecular events to the years of functioning of the human brain.
Are you interested in this position? Please apply using the application button before 15 June 2023. Please do not apply through mail, only applications through the VU application system will be considered.
Your application should include a single pdf file that contains the following:
- motivation letter (1 page)
- your CV (including your education history, short summary of your PhD thesis and other research experience, a list of peer-reviewed publications)
- at least 2 reference contacts
If you have any questions regarding this vacancy, you may contact:
Name: Prof.dr. Huib Mansvelder
Working at VU Amsterdam
Working at Vrije Universiteit Amsterdam means contributing to a better world. Working beyond the confidence of your own field, working with an open mind and working for the benefit of society.
Apply for this job
Apply for this job
This application process is managed by the employer (Vrije Universiteit Amsterdam (VU)). Please contact the employer for questions regarding your application.Apply for this job via the employer's website
Thank you for applying
Please contact the employer for questions regarding your application.
Tip: save this job as favorite in your AcademicTransfer account. This gives you an immediate overview and makes it easy to find the job later on. No account yet? Create it now and take advantage of other useful functionalities too!
Back to the vacancy
Postdoctoral Researcher Modelling Drought Human Dynamics In The Context Of Climate Services, Vrije Universiteit Amsterdam, Netherlands, about 4 hours ago
Are you interested in modelling the feedback between droughts and society? Do you want to contribute to advancing our understanding on the effect of short-term adaptation strategies on long-term d...
Postdoc Position “Ethics Of Precommitment”, Vrije Universiteit Amsterdam, Netherlands, about 4 hours ago
The Department of Philosophy at Vrije Universiteit Amsterdam has a vacancy for a postdoc researcher on a project related to the “Ethics of Precommitment” (3-year position). Location: AMSTERDAM FT...
Postdoc Position In Brillouin Microscopy, Vrije Universiteit Amsterdam, Netherlands, about 4 hours ago
Would you like to be one of the driving forces of a project that aims to develop a new mechanical characterization technique for living cell experiments? If yes, this position may be the one that ...
Postdoctoral Researcher In Neuroscience And/Or Human Stem Cell Based Neuronal Models, UNIVERSITY OF HELSINKI, Finland, 28 days ago
6 Sep 2023 Job Information Organisation/Company UNIVERSITY OF HELSINKI Research Field Medical sciences Psychological sciences Researcher Profile Recognised Researcher (R2) Established Researcher (...
Post Doctoral Fellow In The School Of Biomedical Sciences, THE UNIVERSITY OF HONG KONG, United Kingdom, 24 days ago
Work type: Full-time Department: School of Biomedical Sciences (22600) Categories: Senior Research Staff & Post-doctoral Fellow Applications are invited for appointment as Post-doctoral Fellow in ...
Postdoc (M/F/D) Junior Research Group Muscle Physiology And Metabolism, Leibniz, Germany, 22 days ago
The German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE) is a member of the Leibniz Association. The institute’s mission is to conduct experimental and clinical research in the field of ...