Postdoc (3 yr) Intracellular Notch trafficking and stem cell fate

Maastricht University (UM), Netherlands

Updated: over 2 years ago
Job Type: Temporary
Deadline: 08 Nov 2021

Background: Notch proteins are master regulators of cell fate during development and control cell renewal and differentiation in most adult tissues. Activation of Notch proteins is regulated by successive enzymatic cleavages that release the cell membrane-bound form that acts as a transcriptional regulator. More recently, import roles of intracellular vesicles in regulating Notch activity are being identified (see Pubmed 34572582). We have now identified metal-binding transporters (DMT1) as key mediators of intracellular Notch trafficking and activity.


Key objectives. To identify the importance of metal transport on intracellular Notch localization and activity in vesicles and how this can block Notch activity in tumours or promote regeneration of normal cell types. You will apply gain and loss of function (CRISPR, siRNA pharmacological) in Notch-dependent 2D and 3D models (cell lines, normal and tumour organoids (lung, intestine) and high-resolution fluorescent imaging (confocal, STED) and single-cell analysis to decipher how Dmt1/Notch signaling allocates cell identify and how iron transport is involved. You will use reagents we developed (Ab’s, Tg mouse models) to track the fate of Notch activity in fixed and live tissues.


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