PUBLIC COMPETITION FOR 12 MONTHS TYPE B FELLOWSHIP FOR COLLABORATION TO RESEARCH ACTIVITIES IN THE...
Expertise in research area: previous research experience in the field of immunology, cell biology and molecular biology. Purification and culturing of lymphocytes isolated from tumor tissue and from peripheral blood. Multiparametric cytofluorimetry for phenotypic and functional analysis of tumor infiltrate (cell cycle, intracellular staining on fixed cells, cell viability and apoptosis, proliferation). Molecular biology techniques (Single-cell RNA-Seq, PCR, RT-qPCR, design and implementation of cloning). Phenotypic and functional study of genes and proteins. ELISA and high density array tests for cytokine dosing in biological fluids. Basic microscopy techniques (immuno-fluorescence, IHC). Familiarity with flow cytometry analysis software (FlowJo). Development of cellular assays to study the interaction between immune system and cancer cells.
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