Technical Assistant (MTA/BTA or equivalent) (m/f/d)

25/08/2023

The MPI-CBG is looking for a Technical Assistant (MTA/BTA or equivalent) (m/f/d).

Application Deadline is 22 September 2023

The research group of Dr. Jesse Veenvliet is looking for a

Technical Assistant (MTA/BTA or equivalent) (m/f/d)

working with embryonic organoids, microscopy and genome engineering.

The lab of Jesse Veenvliet focuses on Stembryogenesis (or embryonic organoids), reconstructing mouse and human embryonic development by building embryo-like structures from stem cells in a dish to better understand the complex processes by which embryos shape themselves. The team works with stembryos, because in contrast to their natural counterpart, they are easy to access, track, manipulate and scale, thereby enabling quantitative interrogation of developmental processes across spatial and temporal scales. The diverse “Team Stembryo” is highly interdisciplinary, international and collaborative; English is our institute working language. The successful candidate will be responsible for preparing, performing and post-processing of experiments, initial analysis, recording of data, and general laboratory organisation.

• Conduct experimental tissue culture activities such as mESC/hESC/hiPSC expansion, freezing, generation of embryonic organoids in large numbers.
• Perform experimental molecular biology work such as cloning, CRISPR, DNA/RNA/protein extraction, electrophoresis, western blot, including recording of data.
• Light Microscopy: collect and prepare samples (e.g. tissue clearing), acquire data with wide-field, spinning disk, confocal laser scanning , multi-photon, and light sheet microscopes. Assist with live imaging.
• Assist in troubleshooting procedures (including discussions with in-house facilities) in case of issues with experimental pipelines.
• Lab organisation, ordering and indexing of materials, safety documentation, equipment maintenance.

• Research technician degree (MTA/BTA) or comparable qualification
• Expertise with mouse and human pluripotent stem cell culture (mESC/hESC/hIPSC)
• Expertise with routine molecular biology techniques (e.g. cloning, DNA/RNA/protein extraction, electrophoresis)
• Expertise with confocal microscopy
• Flexibility, team spirit, organisational and interpersonal skills
• Excellent level of written and spoken English

• Experience with CRISPR-Cas9-genome editing and genotyping
• Expertise with library preparation for RNA-sequencing
• Expertise with basic imaging analysis (e.g. Fiji, Imaris, Napari)
• Good level of written and spoken German

• We offer a diversified job in a young, scientifically oriented, international team in one of the most renowned research institutions worldwide;
• Salary and social benefits will be in accordance with the applicable German tariff for employees in civil service (TVöD Bund) corresponding with qualification, experience and job responsibilities;
• Attractive pension plan via VBL (Federal and State Government Employees' Retirement Fund);
• Discounted job ticket (VVO) and good public transport connections;
• Support for a positive work-life balance, including a proactive health programs and assistance with family/childcare matters.

The position is available immediately. The initial contract is for 2 years with the possibility of extension.

The Max Planck Society strives for gender and diversity equality. We welcome applications from all backgrounds. People with disabilities are encouraged to apply.
For initial enquiries, please contact Brian von Rueden, Head of Human Resources, via hr@mpi-cbg.de.

Applications will be reviewed on a rolling basis until the position is filled. We encourage you to submit your complete application by 22 September 2023 via our online application system stating Code 2023-Technician-Stembryogenesis-2470: https://recruitingapp-2443.umantis.com/Vacancies/562/Application/CheckLogin/2?lang=eng

The following materials should be included:

• Cover letter indicating the motivation to join the Veenvliet lab
• CV including publications
• Academic and professional certificates; contact details of approachable references