Senior Scientist / Postdoc (PhD) (m/f/d) CRISPR-mediated gene therapy in lung disease

01.08.2022, Wissenschaftliches Personal [ACHTUNG: im Moment schreibend nicht nutzbar 27.08.2022]

The Research Unit Translational Immunology (TUM) and the Institute of Lung Health and Immunity (LHI; Helmholtz Munich) joined forces to better understand and cure lung diseases associated with a dysregulated STAT3 signaling using CRISPR-mediated gene editing. The proposed project focuses on STAT3 gene correction in lung cancer and STAT3-hyper-IgE syndrome (STAT3-HIES), which is a monogenic inborn error of immunity with chronic destructive lung disease. To correct the underlying disease-causing STAT3 mutations we established CRISPR/Cas9-mediated base editing (Eberherr et al. CRISPRJ). To study pulmonary delivery of gene editing tools in lung cancer cells and iPSC-derived alveolar epithelial cells we are looking for a

Your responsibilities:
 To develop an innovative technique to correct lung diseases as part of an interdisciplinary team
 To perform gene editing of human lung cancer cells and iPSC-derived alveolar epithelial cells
 To assess efficiency and safety of the delivery method and the performed CRISPR-mediated gene editing

Your profile:
 PhD in one of the life sciences (preferably pharmaceutical studies), MD or equivalent work experience in the wet lab
 A strong background in cellular delivery of nucleic acids or proteins including formulation development and cell culture technique is favorable
 Ideally you have experience in gene editing (including gRNA design and validation)
 Experience in downstream analysis including immunofluorescence staining, flow cytometry, western blot analysis, quantitative real-time PCR, Sanger sequencing, high-throughput sequencing, in silico analysis of editing efficiency, cell viability analysis is a plus
 The highly motivated candidate must be able to work independently however we greatly seek team player mentality

We offer you:
 The Technical University of Munich (TUM) is one of the leading research universities in Europe.
 We are offering excellent working conditions and an international research environment.
 The project will be conducted at the Munich-Neuherberg campus of Helmholtz Munich.
 The project is initially limited to two years. An extension of the project is planned.

In general, a full-time position can also be filled on a part-time basis. If the candidates’ suitability for the position in question is equal, severely disabled applicants shall be given preference. Interview-related costs can, unfortunately, not be reimbursed.
We look forward to your application!

Please submit your complete application documents (letter of motivation, CV, (degree) certificates, at least two references) by e-mail quoting number 05_022 by 31.08.2022 at the latest:
Prof. Dr. med. Ellen Renner
Research Unit Translational Immunology (TUM)
Klinikum rechts der Isar der Technischen Universität München
Ismaninger Straße 22
81675 München
E-Mail: [email protected]

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Kontakt: [email protected]

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