Fachbereich Veterinärmedizin - Institut für Veterinär-BiochemieResearch assistant (Praedoc) (m/f/d ) with 65%-part-time job limited to 3 years Entgeltgruppe 13 TV-L FU reference code: WE03-SFRP1 Bewerbungsende: 10.04.2023

Job description:

A position for a Research Assistant (PraeDoc) (m/f/d) TV-L13 (65%)) is available in the laboratory of Dr Torsten Stein at the Institute of Veterinary Biochemistry FU-Berlin, Germany, as part of a collaboration with Prof. Robert Klopfleisch, Institute of Veterinary Pathology. The successful candidate will study the role of the secreted frizzled-related protein 1 (SFRP1) in metastatic and non-metastatic mammary cancers in dogs. The aims of the project are to establish SFRP1 as a biomarker (RNA, protein) for metastasis formation, to identify its associated signalling pathways, and to characterise its function in relation to cell-cell communication and cancer cell behaviour.
This position allows the successful candidate to undertake innovative research as part of an international collaboration to gain a doctorate (Dr. rer. nat./PhD) within the established structured PhD programme of the Dahlem Research School (DRS) Biomedical Sciences. In addition to the valuable transferable skills taught within the programme, the practical work of the project will allow the student to gain deep expertise in a wide range of molecular pathology and biology techniques including immunohistochemistry, RT-qPCR from FFPE material, handling and interpretation of clinical (pathology) data, cell culture (primary cells, cell lines development, 2D and 3D culture), immunofluorescence, in vitro cell migration and invasion studies, CrispR/Cas9 technology, and general molecular biology techniques.

Requirements:
You have a successfully completed university degree in veterinary medicine, biology, biochemistry (Master/Diplom) or equivalent.

Desirable:
Demonstrable practical experience in molecular and cell biology techniques (qPCR, cloning, mammalian cell culture), protein biochemistry or histopathology are desirable. The candidate should be self-motivated, well-organised, show an enthusiasm for the research field, and should be able to work independently as part of a team. Experience in mammary gland biology would be beneficial. The candidate should have excellent skills in spoken and written English.

Applications should be sent by e-mail, together with significant documents, indicating the reference code, in PDF format (preferably as one document) to Dr. Torsten Stein: torsten.stein@fu-berlin.de or postal to

Freie Universität Berlin

Fachbereich Veterinärmedizin

Institut für Veterinär-Biochemie

Herrn Dr. Torsten Stein

Oertzenweg 19 b

14163 Berlin (Düppel)

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