Researcher in antibody molecular engineering

5 Apr 2024
Job Information

Organisation/Company

CEA Saclay

Department

DRF/JOLIOT/DMTS

Research Field

Biological sciences » Biology

Researcher Profile

Established Researcher (R3)

Country

France

Application Deadline

28 Jun 2024 - 12:00 (Europe/Paris)

Type of Contract

Permanent

Job Status

Full-time

Offer Starting Date

2 Sep 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

The Molecular Engineering Services for Health (SIMoS) is part of the CEA's "Medicines and Technologies for Health" department. SIMoS specialises in the development and characterisation of molecules for diagnosis, imaging and therapy, particularly antibodies. The Cellular Immunology and Biotechnology Laboratory (LICB) is developing antibody engineering and epitope mapping methods based on the Yeast Surface Display technique.

Many innovative therapies and diagnostic systems are based on the use of monoclonal antibodies. A large proportion of these molecules are obtained by immunising animals, so their characteristics are highly dependent on the quality of the animals' immune responses. Their affinity may be insufficient or their recognition spectrum poorly controlled. Alternatives for generating antibodies in vitro exist and involve screening synthetic libraries to rapidly identify new molecules with the desired affinity and selectivity properties.

The aim of this post is to implement in vitro antibody generation technologies at SIMoS that do not use animals but synthetic immunoglobulin libraries.

 

Job description

Within the CEA, SIMoS is recruiting a permanent contract researcher in Molecular Engineering of Antibodies and Molecular Biology to strengthen its research activity in the discovery of new antibodies for therapeutic or diagnostic purposes. The objectives of this post are i) to create synthetic libraries of human immunoglobulins and nanobodies (VHH) with controlled diversity and ii) to exploit the libraries generated to develop new molecules of interest for the laboratory's projects, directed against pathogens of emerging diseases or the terrorist threat, as well as against other targets of therapeutic or diagnostic interest. Finally, the aim is also to iii) develop original research as part of an overall strategy to generate antibodies or nanobodies with high affinity and controlled selectivity.

Requirements

Research Field

Biological sciences » Biology

Education Level

PhD or equivalent

Skills/Qualifications

• PhD in Biotechnology/Molecular Biology with a minimum of 2 years' post-doctoral experience
• Have successfully conducted research in the field
• A solid scientific background and knowledge of major advances in antibody engineering and biotechnology
• Proven skills in molecular biology, in the design and screening of libraries using display techniques (Yeast display or other display methods) and in flow cytometry (FACS).
• Mastery of biochemistry, purification and functional characterisation of antibodies (BLI, ELISA) as well as advanced knowledge of NGS mass sequencing technologies and large-scale sequence processing, in particular using scientific programming languages (R, Python, Galaxy).
• Knowledge of MOT regulations is a plus
• Would like to take part in fund-raising and would like to set up a new activity to complement those of the laboratory.
• Enjoys working in a team, supervising technicians and students
• Is able to report regularly on the progress of its work, and is able to present its data in French and English.

Languages

FRENCH

Level

Good

Languages

ENGLISH

Level

Good

Research Field

Biological sciences » Biology

Years of Research Experience

1 - 4

Additional Information
Work Location(s)

Number of offers available

1

Company/Institute

CEA Saclay

Country

France

Geofield

Where to apply

E-mail

[email protected]

Contact

City

Gif sur Yvette

Website

https://joliot.cea.fr/drf/joliot/Pages/Entites_de_recherche/medicaments_technologies_sante/simos.aspx

Street

Bat 152, PC24

Postal Code

91191

STATUS: EXPIRED