Regulations of RNA splicing

31 Jan 2024
Job Information

Organisation/Company

Université d'Evry Val d'Essonne

Research Field

Biological sciences » Biology
Chemistry » Biochemistry
Biological sciences » Biological engineering

Researcher Profile

Recognised Researcher (R2)
Leading Researcher (R4)
First Stage Researcher (R1)
Established Researcher (R3)

Country

France

Application Deadline

14 Mar 2024 - 22:00 (UTC)

Type of Contract

Temporary

Job Status

Full-time

Offer Starting Date

1 Oct 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

The University of Paris-Saclay is looking for a candidate for a thesis on the mechanism of RNA splicing in eukaryotes. The candidate will work in the SABNP laboratory which is dedicated to the study of RNA binding proteins using integrative approaches combining biochemical, structural and functional analyzes (http://sabnp.univ-evry.fr ).

Scientific project:

RNA splicing is a key step in genome expression that is often altered in genetic diseases and cancers (1). However, detailed knowledge of the mechanisms enabling precise splice site recognition is still lacking. In this context, our team studies the interactions between the factors involved in splice site recognition (2, 3).

The student will carry out biochemical and functional analyzes to characterize the interactions between several splicing factors. The sequences targeted by these factors will also be sought by in vitro RNA-protein interaction analyzes and tested in vivo. Finally, the role of these interactions in the splicing of target genes will be investigated in cellular models. All the results will make it possible to propose new modes of splicing regulation.

1. Bonnal, S. C., López-Oreja, I., and Valcárcel, J. (2020) Roles and mechanisms of alternative splicing in cancer - implications for care. Nat. Rev. Clin. Oncol. 17, 457–474

2. Tari, M., Manceau, V., de Matha Salone, J., Kobayashi, A., Pastré, D., and Maucuer, A. (2019) U2AF65 assemblies drive sequence-specific splice site recognition. EMBO Rep. 20, e47604

3. Kobayashi, A., Clément, M.-J., Craveur, P., El Hage, K., Salone, J. de M., Bollot, G., Pastré, D., and Maucuer, A. ( 2022) Identification of a small molecule splicing inhibitor targeting UHM domains. FEBS J. 289, 682–698

Host laboratory and context: The successful candidate will be funded by the SDSV doctoral school and will carry out research within the SABNP laboratory (INSERM UMR1204 research unit) at the University of Evry, and part of the Genopole biocluster for biotechnologies.

Candidate profile: We are looking for candidates who hold (or are in the process of acquiring) a master's degree (or equivalent) in biochemistry, molecular biology or any field of life sciences. Successful candidates must have good organizational skills and be able to work in a team.

Contact and application: For more information, please contact Alexandre Maucuer at alexandre.maucuer_at_inserm.fr. To apply, please provide a complete CV, a cover letter and a copy of the marks obtained in bachelor's and master's degrees.

Position open until: 03/15/2024

Funding category: Contrat doctoral

PHD title: Doctorat en Biologie
PHD Country: France

Requirements
Specific Requirements

Nous recherchons des candidats titulaires (ou en cours d'acquisition) d'un master 2 (ou équivalent) en biochimie, biologie moléculaire ou tout domaine des sciences de la vie. Les candidats retenus doivent avoir de bonnes compétences organisationnelles et savoir travailler en équipe.

Additional Information
Work Location(s)

Number of offers available

1

Company/Institute

Université d'Evry Val d'Essonne

Country

France

City

Evry

Geofield

Where to apply

Website

https://www.abg.asso.fr/fr/candidatOffres/show/id_offre/119815

Contact

Website

http://sabnp.univ-evry.fr

STATUS: EXPIRED